DOT Oral Fluid Collector Training (Includes Proficiency Mocks)

The U.S. Department of Transportation (DOT) has approved and authorized oral fluid drug testing as a method to conduct a DOT required drug test for all modes and DOT drug testing purposes. (88FR 27596) Collection sites, Specimen Collectors, Employers or any persons wishing to perform a DOT oral fluid drug test must be DOT qualified by successfully completing a DOT oral fluid collector training course and complete five monitored DOT proficiency collection demonstrations (mocks) utilizing an HHS approved oral fluid device. Accredited Drug Testing and our training team are one of the first group of trainers certified to train DOT oral fluid collectors and monitor proficiency mock demonstrations to become DOT qualified. As one of the Nation’s leading providers of DOT drug and alcohol testing, and DOT drug and alcohol training programs, we offer four training methods to become qualified as a DOT oral fluid specimen collector. Accredited Drug Testing is an endorsed provider of the National Drug & Alcohol Screening Association. (NDASA)

Click the training program that’s right for you.

Training Program Descriptions

Online DOT Oral Fluid Training (Self Paced)- This training program is comprised of two sections. The online course (4 hours) and upon completion, scheduling your DOT Required Proficiency Mocks, which will be monitored by a scheduled Zoom Call. Once you have purchased the training program, you will receive a confirmation and access link in which you will be able to establish your own login and password. Once you successfully complete both the training and mocks you will receive your certificate of qualification to perform DOT Oral Fluid Specimen Collections. This online training program is designed for those student who have busy schedules and need the ability to start & stop until the program is completed.

Live Classroom DOT Oral Fluid Training- Live training classes will be held monthly in all major U.S. Cities, with training held from 8:00am to 12:00pm, lunch break and then proficiency collection demonstrations (mocks) from 1:00pm to 5:00pm. Click here to view upcoming class schedule including dates and cities. Register online for discount, walk ins welcome at full price if space is available.  Once you successfully complete both the training and mocks you will receive your certificate of qualification to perform DOT Oral Fluid Specimen Collections.


Webinar/Zoom DOT Oral Fluid Training- Webinar text This training program is comprised of two sections. A Webinar based course (4 hours) in which attendees will be trained and have the ability interact via zoom, including asking questions in a chat format and upon completion, scheduling your DOT Required Proficiency Mocks, which will be monitored by a scheduled Zoom Call. Once you have purchased the training program, you will receive a confirmation with a schedule of upcoming webinars (2 per month). Once you select your webinar date, reminders and access links will be provided. Once you successfully complete both the training and mocks you will receive your certificate of qualification to perform DOT Oral Fluid Specimen Collections.

Customized DOT Oral Fluid Training- Accredited Drug Testing can provide customized DOT Oral Fluid Training programs for collection sites, collectors, Human Resources Managers or a DOT Regulated Employers to become qualified to conduct DOT Oral Fluid Drug Testing. These customized program can include onsite training (we come to you), webinar training for in-house groups needing additional training programs such as urine/hair training in addition to DOT Oral Fluid Collector Training. Complete the contact us inquiry form and a member of our training team will contact you within 72 hours.

Read The New Section In 49 CFR Part 40 – Section 40.35

To be permitted to act as an oral fluid collector in the DOT drug testing program, you must meet each of the requirements of 49 CFR Part 40 Section 40.35:


** Training and proficiency mocks are provided in partnership with the National Drug and Alcohol Screening Association (NDASA) and upon successfully completing the training and proficiency mocks, students will receive a Certificate of Qualification issued by both Accredited Drug Testing and NDASA.
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