Urine| Hair Follicle | Oral Fluid | Blood | ETG
Hair analysis is now viewed as an effective method for detecting the usage of substances like drugs and alcohol. Hair chronicles a prolonged history of alcohol and drug consumption by encapsulating biomarkers within the fibers of the maturing hair shaft. When retrieved near the scalp, hair may offer a detection range of up to roughly 90 days for alcohol and drugs. Collection of hair is straightforward, moderately challenging to tamper with, and simple to dispatch.
A 1.5-inch hair sample comprising about 200 hairs (approximately the size of a #2 pencil) closest to the scalp will produce 100mg of hair, which is the optimal sample size for screening and verification. For EtG, additional analyses, and/or tests exceeding 10 panels, 150mg of the hair sample is suggested. Using a jeweler’s scale to weigh the sample is advised. If scalp hair cannot be obtained, an equivalent quantity of body hair can be gathered. The term head hair refers solely to scalp hair, whereas body hair encompasses all other hair types (facial, axillary, etc.).
Process Overview
The primary phases in processing a drug test outcome in a laboratory setting are Accessioning, Screening, Extraction, and Confirmation.
Accessioning pertains to the preliminary handling of a sample within a lab’s infrastructure. This process involves ensuring the sample was sealed and sent appropriately, assigning a unique LAN (Laboratory Accessioning Number), and fulfilling any extra data input not furnished by an electronic chain of custody system.
Screening carries out an initial rapid assessment for substances commonly misused. Although Screening is a budget-friendly technique to eliminate drug use in most cases, a positive screen needs confirmation for legal recognition. Every sample yielding a tentative positive in Screening necessitates further verification.
If a sample appears positive during the Screening phase, additional hair is extracted from the original sample and readied for Extraction. At this stage, drugs are isolated from hair at concentrations notably lower than found with other methods (such as urine or oral fluid), thus making hair drug analysis the most complex technique.
The positive results from screening are validated through GC/MS, GC/MS/MS, or LC/MS/MS. All samples showing positive presumptive results are cleansed prior to confirmation as required. The comprehensive laboratory procedure from Accessioning to Confirmation undergoes scrutiny under the CAP (College of American Pathologists) Hair certification and aligns with the ISO / IEC 17025 standards.
Advantages of hair drug testing:
Limitations:
Note: Although frequently termed "hair follicle tests", this test examines the hair strand itself, not the follicle beneath the scalp.
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