✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Nashville, TN

With over 30 testing centers in the Nashville area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

34 Drug-Testing Centers in Nashville

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

3441 Dickerson Pike Outpatient Lab

3.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3441 Dickerson Pike Outpatient Lab
Nashville, TN 37207

3638 Dickerson Pike Ste 204

4.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3638 Dickerson Pike Ste 204
Nashville, TN 37207

1209 S Graycroft Ave

5.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1209 S Graycroft Ave
Madison, TN 37115

360 Deaderick St

5.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
360 Deaderick St
Nashville, TN 37201

315 14th Ave

5.84 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-7:00 PM • Sat: 9:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
315 14th Ave
Nashville, TN 37203

1719 Gallatin Ave

5.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1719 Gallatin Ave
Nashville, TN 37206

1916 Patterson St Ste 103,

5.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1916 Patterson St Ste 103,
Nashville, TN 37203

300 20th Ave N

5.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 20th Ave N
Nashville, TN 37203

2010 Church St 1st Fl

6.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2010 Church St 1st Fl
Nashville, TN 37236

602 Gallatin Ave

6.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
602 Gallatin Ave
Nashville, TN 37206

339 White Bridge Pike

6.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
339 White Bridge Pike
Nashville, TN 37209

114a 29th Ave N

6.46 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
114a 29th Ave N
Nashville, TN 37203

3404 W End Ave Ste 101

6.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3404 W End Ave Ste 101
Nashville, TN 37203

6606 Charlotte Pike Ste 104

7.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6606 Charlotte Pike Ste 104
Nashville, TN 37209

2800 Opryland Drive

7.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2800 Opryland Drive
Nashville, TN 37214

1616 Gallatin Pike N

8.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1616 Gallatin Pike N
Madison, TN 37115

1719 Gallatin Pike N

8.50 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1719 Gallatin Pike N
Madison, TN 37115

1425 Elm Hill Pike

8.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1425 Elm Hill Pike
Nashville, TN 37210

2001 Glen Echo Rd

8.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2001 Glen Echo Rd
Nashville, TN 37215

1000 Northchase Dr Ste 215

9.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1000 Northchase Dr Ste 215
Goodlettsville, TN 37072

2372 Lebanon Pike

9.66 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat: 8:00 AM-6:00 PM • Sun: 1:00 PM-5:00 PM✓ Walk-in with Electronic Donor Pass
2372 Lebanon Pike
Nashville, TN 37214

2531 Elm Hill Pike

10.43 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-5:00 PM • Sat: 9:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
2531 Elm Hill Pike
Nashville, TN 37214

Po Box 140294

10.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Po Box 140294
Nashville, TN 37214

4300 Sidco Dr

10.73 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
4300 Sidco Dr
Nashville, TN 37204

3656 Trousdale Dr Ste 109

10.80 mi
🕑 Testing Hours: M-Th 8:00 AM-12:00 PM & 1:00 PM-4:00 PM • F 8:00 AM-12:00 PM & 1:00 PM-2:30 PM✓ Walk-in with Electronic Donor Pass
3656 Trousdale Dr Ste 109
Nashville, TN 37204

2740 Old Elm Hill Pike Ste 103a

10.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2740 Old Elm Hill Pike Ste 103a
Nashville, TN 37214

519 Donelson Pike Ste 107

11.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
519 Donelson Pike Ste 107
Nashville, TN 37214

3055 Lebanon Pike Ste 1000

11.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3055 Lebanon Pike Ste 1000
Nashville, TN 37214

2267 Jackson Downs Blvd

11.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2267 Jackson Downs Blvd
Nashville, TN 37214

7643 Highway 70 S

11.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7643 Highway 70 S
Nashville, TN 37221

501 Metroplex Dr Ste 306

12.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
501 Metroplex Dr Ste 306
Nashville, TN 37211

391 Wallace Rd

12.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
391 Wallace Rd
Nashville, TN 37211

5225 Old Hickory Blvd Ste 205

12.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5225 Old Hickory Blvd Ste 205
Hermitage, TN 37076

1645 Murfreesboro Pike Ste H

12.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1645 Murfreesboro Pike Ste H
Nashville, TN 37217

Employment, DOT, Court Ordered, Probation, Family

Accredited Drug Testing provides a wide range of drug and alcohol testing services at our 34 testing locations around Nashville, Tennessee. Our offerings include both DOT and non-DOT urine tests, breathalyzer tests, EtG alcohol testing, and hair drug analysis, catering to individuals, companies, and legal requirements. In Nashville, TN, rapid result testing and SAMSA certified laboratory analyses are available with same-day service in most cases. Our Nashville centers are conveniently located close to your home or office. We also offer Occupational Health Testing, Clinical Testing, and conduct Background Checks.

Contact us at (800) 221-4291 or register online. Choose a test and location that suits you—whether it's for personal reasons, for your employees, or someone else. Scheduling is quick and easy; connect with our scheduling team or arrange your test online anytime. Our efficient process makes organizing drug testing near Nashville smooth and straightforward.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Nashville, TN

1

Order Online or Call

Select your test & search nearby Nashville collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Nashville, TN Drug Testing

Drug Testing Services Available at Our Nashville, TN Labs:

At our Nashville drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Tennessee Drug Testing Laws & Compliance in Nashville

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Nashville, TN adhering strictly to T.C.A. § 50-9-101 et seq., federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
T.C.A. § 50-9-101 et seq.
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Tennessee Drug-Free Workplace Program provides comprehensive employer defense protections and worker compensation premium discounts for certified testing.

Under T.C.A. § 50-9-101 et seq., employers in Nashville may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Tennessee), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Nashville, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Nashville and Tennessee.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Nashville, TN

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Nashville to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Nashville Neighborhoods Covered:

● Downtown Nashville ● The Gulch ● Music Row ● East Nashville ● Germantown ● Green Hills ● 12 South ● Midtown ● SoBro ● West End ● Donelson

Major Employment & Commercial Hubs Served:

Downtown Healthcare & Financial Hub • Music Row Entertainment District • MetroCenter Business Park • BNA Airport Logistics Hub

Primary Nashville ZIP Codes Served:

37201 37203 37206 37208 37212 37215 37219
Located anywhere in Nashville? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Nashville

Metropolitan Government • Nashville-davidson • Berry Hill • Goodlettsville • Milltown • Belle Meade • Oak Hill • Lakewood

Nashville, TN Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Nashville.

Test Type & Local Panel Detection Window Common Use Cases in Nashville Turnaround Time
5-Panel Urine Screen in Nashville →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Nashville →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Nashville →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Nashville →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Nashville →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Nashville →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Nashville, TN

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Nashville.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Nashville facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Nashville, TN

Need immediate walk-in service in Nashville? View our Walk-In Drug Testing Near Me in Nashville, TN facility guide or order an instant electronic donor pass online.

Nashville Drug Testing

Quickly find trusted local drug testing centers in Nashville, TN — fast, convenient, and reliable every time!

Nashville DOT Drug Testing

Quickly find a local DOT drug testing center in Nashville, TN — fast, reliable, convenient nationwide service!

Nashville DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Nashville, TN.

Nashville Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Nashville, TN.v

Nashville Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Nashville, TN locations—results you can trust, every time!

Nashville Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Nashville, TN.

Nashville Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Nashville, TN.

Nashville 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Nashville, TN employers and individuals nationwide.

Nashville 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Nashville, TN workplace and personal testing needs.

Nashville On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Nashville, TN workplace compliant.

Nashville DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Nashville, TN drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Tennessee, workplace drug testing is governed by statutory guidelines under T.C.A. § 50-9-101 et seq.. Private employers generally operate under a Tennessee Drug-Free Workplace Program, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Tennessee who implement a certified Drug-Free Workplace Program in compliance with T.C.A. § 50-9-101 et seq. can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Tennessee state law compliance standards, state statutes allows voluntary testing with workers compensation premium discounts. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 34 certified collection sites in the Nashville area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Nashville, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Friendly and Knowledgeable

I reached out Accredited Drug Testing/Secure Screenings to partner for my drug testing business (Discovery Diagnostics Columbia SC) and the process was extremely easy. I worked with Sicilia who was amazing. She was friendly & knowledgeable, and answer all of my questions with ease.

Kendra Harley - 3/26/2025

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025

Super helpful

Customer service was surprisingly super helpful and so nice. She was able to get me in at a local location right away the same day. She helped me figure out exactly what I needed. Very impressed.

Brook - 9/19/2024


(800) 221-4291