✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Gatlinburg, TN

With 40 testing centers in the Gatlinburg area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

40 Drug-Testing Centers in Gatlinburg

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

675 Middle Creek Rd

0.71 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-2:00 PM • Sat: 8:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
675 Middle Creek Rd
Sevierville, TN 37862

601 Wall St

1.48 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-11:30 AM & 1:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
601 Wall St
Sevierville, TN 37862

300 Prince St

1.97 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-11:30 AM & 12:00 PM-1:30 PM✓ Walk-in with Electronic Donor Pass
300 Prince St
Sevierville, TN 37862

1815 Parkway

2.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1815 Parkway
Sevierville, TN 37862

1548 Parkway Ste 201

2.16 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-12:00 PM & 1:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
1548 Parkway Ste 201
Sevierville, TN 37862

441 Parkway Ste 2

2.16 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-11:30 AM & 12:00 PM-1:30 PM✓ Walk-in with Electronic Donor Pass
441 Parkway Ste 2
Sevierville, TN 37862

240 Forks Of The River Pkwy

2.32 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-3:20 PM • Sat: 6:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
240 Forks Of The River Pkwy
Sevierville, TN 37862

1787 Veterans Blvd Ste 101

2.81 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1787 Veterans Blvd Ste 101
Sevierville, TN 37862

2190 Winfield Dunn Pkwy

5.84 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2190 Winfield Dunn Pkwy
Sevierville, TN 37876

3030 Easy St

6.51 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
3030 Easy St
Sevierville, TN 37862

3222 Chestnut Hill School Rd

11.74 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3222 Chestnut Hill School Rd
Dandridge, TN 37725

1026 Highway 92 S

12.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1026 Highway 92 S
Dandridge, TN 37725

1015 Hunters Xing

14.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1015 Hunters Xing
Alcoa, TN 37701

8712 Asheville Hwy

16.34 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM✓ Walk-in with Electronic Donor Pass
8712 Asheville Hwy
Knoxville, TN 37924

110 Hospital Dr

17.10 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM • Sat: 8:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
110 Hospital Dr
Jefferson City, TN 37760

7200 Strawberry Plains Pike

17.12 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:30 AM, 12:30 PM - 02:00 PM
7200 Strawberry Plains Pike
Knoxville, TN 37914

153 E Broadway Blvd

17.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
153 E Broadway Blvd
Jefferson City, TN 37760

2725 E Governor John Sevier Hwy

18.03 mi
🕑 Facility: Mon–Fri: 07:00 AM - 02:30 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 02:30 PM
2725 E Governor John Sevier Hwy
Knoxville, TN 37914

1171 Highway 11 E Ste 101

18.23 mi
🕑 Facility: Mon–Fri: 07:30 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 04:00 PM
1171 Highway 11 E Ste 101
Talbott, TN 37877

407 4th St

20.54 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM • Sat: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
407 4th St
Newport, TN 37821

434 4th St Ste 310

20.58 mi
🕑 Testing Hours: Mon–Fri: 10:00 AM-2:30 PM • Sat: 7:30 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
434 4th St Ste 310
Newport, TN 37821

4306 Asheville Hwy

21.49 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 02:30 PM
4306 Asheville Hwy
Knoxville, TN 37914

1403 State St

22.11 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM⚡ Drug Screens: Mon–Fri: 11:00 AM - 02:00 PM
1403 State St
White Pine, TN 37890

1409 State St

22.11 mi
🕑 Facility: Mon–Fri: 06:00 AM - 01:30 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 12:30 PM
1409 State St
White Pine, TN 37890

4711 Centerline Dr

23.23 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:00 AM, 01:00 PM - 03:00 PM
4711 Centerline Dr
Knoxville, TN 37917

1932 Alcoa Hwy, Bldg C,#155

24.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1932 Alcoa Hwy, Bldg C,#155
Knoxville, TN 37920

1826 Ailor Ave

24.19 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1826 Ailor Ave
Knoxville, TN 37921

930 E Emerald Ave Ste 813

24.20 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM (Lunch: 12:30 PM - 01:00 PM)✓ Walk-in with Electronic Donor Pass
930 E Emerald Ave Ste 813
Knoxville, TN 37917

1924 Alcoa Hwy Ste U67

24.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1924 Alcoa Hwy Ste U67
Knoxville, TN 37920

2270 Sutherland Ave Ste 101

24.74 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2270 Sutherland Ave Ste 101
Knoxville, TN 37919

2547 Sutherland Ave

25.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2547 Sutherland Ave
Knoxville, TN 37919

1006 E Lamar Alexander Pkwy

25.12 mi
🕑 Facility: Mon–Fri: 06:30 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 03:30 PM
1006 E Lamar Alexander Pkwy
Maryville, TN 37804

117 Gill St

25.68 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
117 Gill St
Alcoa, TN 37701

207 Gill St

25.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
207 Gill St
Alcoa, TN 37701

266 Joule St

26.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
266 Joule St
Alcoa, TN 37701

4028 Papermill Dr Ste 9

26.18 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 08:30 AM - 11:30 AM, 01:30 PM - 04:30 PM
4028 Papermill Dr Ste 9
Knoxville, TN 37909

2412 W Andrew Johnson Hwy

26.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2412 W Andrew Johnson Hwy
Morristown, TN 37814

220 Associates Blvd

26.41 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
220 Associates Blvd
Alcoa, TN 37701

4206 Sutherland Avenue

26.45 mi
🕑 Facility: Mon–Fri: 07:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:30 AM, 01:30 PM - 04:00 PM
4206 Sutherland Avenue
Knoxville, TN 37919

4605 Papermill Dr Ste 1

26.62 mi
🕑 Facility: Mon–Fri: 06:30 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 06:30 AM - 03:30 PM
4605 Papermill Dr Ste 1
Knoxville, TN 37909

Employment, DOT, Court Ordered, Probation, Family

Accredited Drug Testing delivers extensive drug and alcohol testing services across our 40 locations in the Gatlinburg, Tennessee area. We cater to DOT and non-DOT requirements with urine drug evaluations, breathalyzer alcohol screenings, EtG alcohol checks, and hair drug assessments. Services accommodate individuals, workplace testers, and legal demands, offering quick result options and SAMSA certified analyses. Most testing centers in Gatlinburg, TN are conveniently positioned close to your residence or office, with same-day services available. Additional offerings include Occupational Health Services, Clinical Tests, and Background Screening.

Dial (800) 221-4291 or visit our website for online registration. Easily pick your specific test and a suitable nearby location—services are provided for personal use, employee checks, or third parties. Organizing a test is swift and straightforward; use our scheduling team or opt for the 24/7 online appointment system. Our efficient and accessible service facilitates smooth drug testing arrangements near Gatlinburg.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Gatlinburg, TN

1

Order Online or Call

Select your test & search nearby Gatlinburg collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Gatlinburg, TN Drug Testing

Drug Testing Services Available at Our Gatlinburg, TN Labs:

At our Gatlinburg drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Tennessee Drug Testing Laws & Compliance in Gatlinburg

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Gatlinburg, TN adhering strictly to T.C.A. § 50-9-101 et seq., federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
T.C.A. § 50-9-101 et seq.
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Tennessee Drug-Free Workplace Program provides comprehensive employer defense protections and worker compensation premium discounts for certified testing.

Under T.C.A. § 50-9-101 et seq., employers in Gatlinburg may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Tennessee), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Gatlinburg, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Gatlinburg and Tennessee.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Gatlinburg, TN

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Gatlinburg to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Gatlinburg Neighborhoods Covered:

● Downtown Gatlinburg ● Central Business District ● North Gatlinburg Commercial Corridor ● South Gatlinburg Industrial Park ● East Gatlinburg Metro Area ● West Gatlinburg Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Gatlinburg Main Street Commercial District • Gatlinburg Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Gatlinburg? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Gatlinburg

Pigeon Forge • Sevierville • Fairgarden • Fair Garden • Knob Creek • Wear Valley • Beech Springs • Dunn Creek

Gatlinburg, TN Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Gatlinburg.

Test Type & Local Panel Detection Window Common Use Cases in Gatlinburg Turnaround Time
5-Panel Urine Screen in Gatlinburg →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Gatlinburg →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Gatlinburg →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Gatlinburg →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Gatlinburg →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Gatlinburg →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Gatlinburg, TN

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Gatlinburg.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Gatlinburg facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Gatlinburg, TN

Need immediate walk-in service in Gatlinburg? View our Walk-In Drug Testing Near Me in Gatlinburg, TN facility guide or order an instant electronic donor pass online.

Gatlinburg Drug Testing

Quickly find trusted local drug testing centers in Gatlinburg, TN — fast, convenient, and reliable every time!

Gatlinburg DOT Drug Testing

Quickly find a local DOT drug testing center in Gatlinburg, TN — fast, reliable, convenient nationwide service!

Gatlinburg DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Gatlinburg, TN.

Gatlinburg Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Gatlinburg, TN.v

Gatlinburg Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Gatlinburg, TN locations—results you can trust, every time!

Gatlinburg Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Gatlinburg, TN.

Gatlinburg Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Gatlinburg, TN.

Gatlinburg 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Gatlinburg, TN employers and individuals nationwide.

Gatlinburg 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Gatlinburg, TN workplace and personal testing needs.

Gatlinburg On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Gatlinburg, TN workplace compliant.

Gatlinburg DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Gatlinburg, TN drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Tennessee, workplace drug testing is governed by statutory guidelines under T.C.A. § 50-9-101 et seq.. Private employers generally operate under a Tennessee Drug-Free Workplace Program, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Tennessee who implement a certified Drug-Free Workplace Program in compliance with T.C.A. § 50-9-101 et seq. can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Tennessee state law compliance standards, state statutes allows voluntary testing with workers compensation premium discounts. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 40 certified collection sites in the Gatlinburg area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Gatlinburg, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Excellent service!

Excellent service! Trish went above and beyond to find me a testing site as close to my home as possible. Results were faster than expected, as well. Thanks for your help, Trish and Accredited!

Es Hache - 4/13/2025

Reception is kind and helpful

Reception is kind and helpful , everything quick and simple , best urgent care

Yusuf Narmuradov - 11/19/2024

Fast and efficient service

Fast and efficient service for employers wanting to do pre employment drug screening that meets DOT requirements!!

Mary Thomas - 4/5/2025


(800) 221-4291