✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Charlotte, TN

With 36 testing centers in the Charlotte area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

36 Drug-Testing Centers in Charlotte

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

250 Beasley Dr

8.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
250 Beasley Dr
Dickson, TN 37055

127 Crestview Park Dr

9.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
127 Crestview Park Dr
Dickson, TN 37055

1904 Highway 46 S Ste 3

11.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1904 Highway 46 S Ste 3
Dickson, TN 37055

432 Highway 46 S

12.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
432 Highway 46 S
Dickson, TN 37055

313 N Main St

16.74 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
313 N Main St
Ashland City, TN 37015

202 N Main St Ste 4

16.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
202 N Main St Ste 4
Ashland City, TN 37015

202 N Main St

16.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
202 N Main St
Ashland City, TN 37015

155 W Front St P.o. Box 609

18.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
155 W Front St P.o. Box 609
Erin, TN 37061

2727 Fairview Blvd

19.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2727 Fairview Blvd
Fairview, TN 37062

2302 Madison St

23.18 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2302 Madison St
Clarksville, TN 37043

1763 Madison St

23.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1763 Madison St
Clarksville, TN 37043

111 Ussery Rd

23.74 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
111 Ussery Rd
Clarksville, TN 37043

311 Landrum Pl Ste 300

23.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
311 Landrum Pl Ste 300
Clarksville, TN 37043

1836 Memorial Dr

23.79 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1836 Memorial Dr
Clarksville, TN 37043

523 Madison St Ste A

24.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
523 Madison St Ste A
Clarksville, TN 37040

110 Main St

24.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
110 Main St
Clarksville, TN 37040

7643 Highway 70 S

24.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7643 Highway 70 S
Nashville, TN 37221

6606 Charlotte Pike Ste 104

25.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6606 Charlotte Pike Ste 104
Nashville, TN 37209

351 Dover Rd

26.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
351 Dover Rd
Clarksville, TN 37042

301 West Main Street

26.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
301 West Main Street
Waverly, TN 37185

601 Beech St

26.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
601 Beech St
Centerville, TN 37033

339 White Bridge Pike

26.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
339 White Bridge Pike
Nashville, TN 37209

2257 Wilma Rudolph Blvd Ste D

27.10 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
2257 Wilma Rudolph Blvd Ste D
Clarksville, TN 37040

2320 Wilma Rudolph Blvd

27.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2320 Wilma Rudolph Blvd
Clarksville, TN 37040

2509 Wilma Rudolph Blvd Ste D

27.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2509 Wilma Rudolph Blvd Ste D
Clarksville, TN 37040

751 Chesapeake Ln Ste 103,

27.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
751 Chesapeake Ln Ste 103,
Clarksville, TN 37040

711 Holly Ln

27.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
711 Holly Ln
Waverly, TN 37185

2339 Hillsboro Rd

28.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2339 Hillsboro Rd
Franklin, TN 37069

651 Dunlop Ln

28.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
651 Dunlop Ln
Clarksville, TN 37040

150 E Swan St

28.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
150 E Swan St
Centerville, TN 37033

2831 Wilma Rudolph Blvd

28.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2831 Wilma Rudolph Blvd
Clarksville, TN 37040

3404 W End Ave Ste 101

29.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3404 W End Ave Ste 101
Nashville, TN 37203

114a 29th Ave N

29.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
114a 29th Ave N
Nashville, TN 37203

2001 Glen Echo Rd

29.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2001 Glen Echo Rd
Nashville, TN 37215

451 Highway 13 S Attn Lab

29.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
451 Highway 13 S Attn Lab
Waverly, TN 37185

1916 Patterson St Ste 103,

30.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1916 Patterson St Ste 103,
Nashville, TN 37203

Employment, DOT, Court Ordered, Probation, Family

Accredited Drug Testing delivers all-encompassing drug and alcohol screening solutions through our 36 testing sites around Charlotte, Tennessee. Our offerings include both DOT and non-DOT urine tests, breath alcohol tests, EtG alcohol tests, as well as hair drug assessments for individuals, organizations, and legal purposes. In Charlotte, TN, we ensure swift result delivery and provide SAMSA certified lab analysis; same-day services are accessible, with most Charlotte testing sites conveniently located near residences or businesses. We also cover Occupational Health Testing, Clinical Testing, and Background Verification.

Dial (800) 221-4291 or register via our online platform. Choose your test type and select a nearby center—testing is available for personal use, employers, or others. Scheduling is quick and straightforward; contact our scheduling team or arrange your test online anytime. Our efficient and intuitive system simplifies organizing drug testing near Charlotte with ease.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Charlotte, TN

1

Order Online or Call

Select your test & search nearby Charlotte collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Charlotte, TN Drug Testing

Drug Testing Services Available at Our Charlotte, TN Labs:

At our Charlotte drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Tennessee Drug Testing Laws & Compliance in Charlotte

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Charlotte, TN adhering strictly to T.C.A. § 50-9-101 et seq., federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
T.C.A. § 50-9-101 et seq.
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Tennessee Drug-Free Workplace Program provides comprehensive employer defense protections and worker compensation premium discounts for certified testing.

Under T.C.A. § 50-9-101 et seq., employers in Charlotte may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Tennessee), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Charlotte, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Charlotte and Tennessee.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Charlotte, TN

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Charlotte to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Charlotte Neighborhoods Covered:

● Downtown Charlotte ● Central Business District ● North Charlotte Commercial Corridor ● South Charlotte Industrial Park ● East Charlotte Metro Area ● West Charlotte Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Charlotte Main Street Commercial District • Charlotte Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Charlotte? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Charlotte

Cumberland Furnace • Vanleer • Sneedville • Dickson • White Bluff • Burns • Tennessee City • Slayden

Charlotte, TN Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Charlotte.

Test Type & Local Panel Detection Window Common Use Cases in Charlotte Turnaround Time
5-Panel Urine Screen in Charlotte →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Charlotte →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Charlotte →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Charlotte →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Charlotte →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Charlotte →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Charlotte, TN

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Charlotte.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Charlotte facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Charlotte, TN

Need immediate walk-in service in Charlotte? View our Walk-In Drug Testing Near Me in Charlotte, TN facility guide or order an instant electronic donor pass online.

Charlotte Drug Testing

Quickly find trusted local drug testing centers in Charlotte, TN — fast, convenient, and reliable every time!

Charlotte DOT Drug Testing

Quickly find a local DOT drug testing center in Charlotte, TN — fast, reliable, convenient nationwide service!

Charlotte DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Charlotte, TN.

Charlotte Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Charlotte, TN.v

Charlotte Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Charlotte, TN locations—results you can trust, every time!

Charlotte Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Charlotte, TN.

Charlotte Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Charlotte, TN.

Charlotte 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Charlotte, TN employers and individuals nationwide.

Charlotte 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Charlotte, TN workplace and personal testing needs.

Charlotte On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Charlotte, TN workplace compliant.

Charlotte DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Charlotte, TN drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Tennessee, workplace drug testing is governed by statutory guidelines under T.C.A. § 50-9-101 et seq.. Private employers generally operate under a Tennessee Drug-Free Workplace Program, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Tennessee who implement a certified Drug-Free Workplace Program in compliance with T.C.A. § 50-9-101 et seq. can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Tennessee state law compliance standards, state statutes allows voluntary testing with workers compensation premium discounts. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 36 certified collection sites in the Charlotte area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Charlotte, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

Excellant Performance

The visit here is always the best . The place is always really clean. The employees are super courteous, very polite, and professional. This is the only drug lab I like to go do my drug and alcohol test. I would like to tell them thank you so much for thier excellent performance and job

Eli Gonzalez - 1/4/2025

Accredited has been wonderful to work with!

Accredited has been wonderful to work with! My court ordered tests are on a very strange schedule but every time I call, they are quick to schedule me and are always able to find a location that works for where I’m at and when! Thank you for making this rather undesirable situation less stressful and efficient.

Madison Sanderford - 8/19/2024


(800) 221-4291