✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Sioux Falls, SD

With 30 testing centers in the Sioux Falls area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

30 Drug-Testing Centers in Sioux Falls

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

1200 S 7th Ave

1.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1200 S 7th Ave
Sioux Falls, SD 57105

Po Box 1840

1.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Po Box 1840
Sioux Falls, SD 57101

1305 W 18th St

2.36 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1305 W 18th St
Sioux Falls, SD 57117

1100 S Euclid Ave

2.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1100 S Euclid Ave
Sioux Falls, SD 57105

900 E 54th St N Ste 200

3.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
900 E 54th St N Ste 200
Sioux Falls, SD 57104

4928 N Cliff Ave

3.54 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4928 N Cliff Ave
Sioux Falls, SD 57104

2221 W Russell St

3.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2221 W Russell St
Sioux Falls, SD 57104

8100 E 38th St

4.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8100 E 38th St
Sioux Falls, SD 57110

5027 S Bur Oak Pl

4.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5027 S Bur Oak Pl
Sioux Falls, SD 57108

6701 S Louise Ave

5.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6701 S Louise Ave
Sioux Falls, SD 57108

6000 W 41st St

5.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6000 W 41st St
Sioux Falls, SD 57106

1101 E Holly Blvd

6.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1101 E Holly Blvd
Brandon, SD 57005

220 S Cliff Ave Ste 120

8.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
220 S Cliff Ave Ste 120
Harrisburg, SD 57032

108 S Main St Attn Lab

17.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
108 S Main St Attn Lab
Lennox, SD 57039

440 N Hiawatha Dr

18.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
440 N Hiawatha Dr
Canton, SD 57013

1600 N Kniss Ave

25.80 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1600 N Kniss Ave
Luverne, MN 56156

803 S Greene St

27.91 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 03:30 PM
803 S Greene St
Rock Rapids, IA 51246

315 1st Ave Ste 208

28.25 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:30 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 12:30 PM, 01:30 PM - 04:00 PM
315 1st Ave Ste 208
Rock Rapids, IA 51246

1202 21st Ave

31.82 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:30 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 11:30 AM, 01:00 PM - 03:00 PM
1202 21st Ave
Rock Valley, IA 51247

206 S Veterans St

34.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
206 S Veterans St
Flandreau, SD 57028

214 N Prairie St

34.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
214 N Prairie St
Flandreau, SD 57028

911 5th Ave Sw

35.84 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-7:00 PM✓ Walk-in with Electronic Donor Pass
911 5th Ave Sw
Pipestone, MN 56164

916 4th Ave Sw

35.86 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
916 4th Ave Sw
Pipestone, MN 56164

903 N Washington Ave

37.80 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
903 N Washington Ave
Madison, SD 57042

120 Nw 2nd St

38.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
120 Nw 2nd St
Madison, SD 57042

1111 11th St

39.70 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1111 11th St
Hawarden, IA 51023

804 S Walnut St

41.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
804 S Walnut St
Freeman, SD 57029

38 19th St Sw

43.01 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 10:00 AM - 03:30 PM
38 19th St Sw
Sioux Center, IA 51250

600 9th Ave N

49.29 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM✓ Walk-in with Electronic Donor Pass
600 9th Ave N
Sibley, IA 51249

118 N 7th Ave

49.68 mi
🕑 Facility: Mon–Fri: 06:30 AM - 04:00 PM✓ Walk-in with Electronic Donor Pass
118 N 7th Ave
Sheldon, IA 51201

Employment, DOT, Court Ordered, Probation, Family

At our 30 testing locations in Sioux Falls, SD, Accredited Drug Testing provides a broad range of drug and alcohol tests. Services include both DOT and non-DOT urine drug screens, breath alcohol tests, EtG alcohol testing, and hair drug analysis tailored for personal, work-related, and legal purposes. With rapid test results and certified laboratory analysis, same-day services are typically available—majority of locations are conveniently situated near homes or offices. Also offered are Occupational Health Testing, Clinical Testing, and conducting Background Checks.

Contact us at (800) 221-4291 or register online today. Choose your desired test and a convenient location nearby—suitable for personal, employee, or third-party testing. Scheduling is quick and hassle-free through our scheduling team or by using our online system any time day or night. Our efficient process ensures easy coordination for drug testing near Sioux Falls.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Sioux Falls, SD

1

Order Online or Call

Select your test & search nearby Sioux Falls collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Sioux Falls, SD Drug Testing

Drug Testing Services Available at Our Sioux Falls, SD Labs:

At our Sioux Falls drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

South Dakota Drug Testing Laws & Compliance in Sioux Falls

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Sioux Falls, SD adhering strictly to S.D.C.L. § 62-4-37, federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
S.D.C.L. § 62-4-37
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

South Dakota law allows employer workplace drug testing and denies workers compensation coverage when workplace injuries stem from willful drug/alcohol misuse.

Under S.D.C.L. § 62-4-37, employers in Sioux Falls may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in South Dakota), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Sioux Falls, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Sioux Falls and South Dakota.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Sioux Falls, SD

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Sioux Falls to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Sioux Falls Neighborhoods Covered:

● Downtown Sioux Falls ● Central Business District ● North Sioux Falls Commercial Corridor ● South Sioux Falls Industrial Park ● East Sioux Falls Metro Area ● West Sioux Falls Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Sioux Falls Main Street Commercial District • Sioux Falls Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Sioux Falls? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Sioux Falls

Pine Lakes Addition • Meadow View Addition • Shindler • Renner Corner • Brandon • Rowena • Harrisburg • Crooks

Sioux Falls, SD Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Sioux Falls.

Test Type & Local Panel Detection Window Common Use Cases in Sioux Falls Turnaround Time
5-Panel Urine Screen in Sioux Falls →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Sioux Falls →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Sioux Falls →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Sioux Falls →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Sioux Falls →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Sioux Falls →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Sioux Falls, SD

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Sioux Falls.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Sioux Falls facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Sioux Falls, SD

Need immediate walk-in service in Sioux Falls? View our Walk-In Drug Testing Near Me in Sioux Falls, SD facility guide or order an instant electronic donor pass online.

Sioux Falls Drug Testing

Quickly find trusted local drug testing centers in Sioux Falls, SD — fast, convenient, and reliable every time!

Sioux Falls DOT Drug Testing

Quickly find a local DOT drug testing center in Sioux Falls, SD — fast, reliable, convenient nationwide service!

Sioux Falls DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Sioux Falls, SD.

Sioux Falls Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Sioux Falls, SD.v

Sioux Falls Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Sioux Falls, SD locations—results you can trust, every time!

Sioux Falls Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Sioux Falls, SD.

Sioux Falls Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Sioux Falls, SD.

Sioux Falls 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Sioux Falls, SD employers and individuals nationwide.

Sioux Falls 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Sioux Falls, SD workplace and personal testing needs.

Sioux Falls On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Sioux Falls, SD workplace compliant.

Sioux Falls DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Sioux Falls, SD drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In South Dakota, workplace drug testing is governed by statutory guidelines under S.D.C.L. § 62-4-37. Private employers generally operate under a South Dakota Codified Laws Title 62, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in South Dakota who implement a certified Drug-Free Workplace Program in compliance with S.D.C.L. § 62-4-37 can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under South Dakota state law compliance standards, state statutes permits zero-tolerance workplace drug testing rules. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 30 certified collection sites in the Sioux Falls area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Sioux Falls, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

Excellant Performance

The visit here is always the best . The place is always really clean. The employees are super courteous, very polite, and professional. This is the only drug lab I like to go do my drug and alcohol test. I would like to tell them thank you so much for thier excellent performance and job

Eli Gonzalez - 1/4/2025

Accredited has been wonderful to work with!

Accredited has been wonderful to work with! My court ordered tests are on a very strange schedule but every time I call, they are quick to schedule me and are always able to find a location that works for where I’m at and when! Thank you for making this rather undesirable situation less stressful and efficient.

Madison Sanderford - 8/19/2024


(800) 221-4291