✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Middletown, PA

With 31 testing centers in the Middletown area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

31 Drug-Testing Centers in Middletown

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

8170 Adams Dr Ste 100 Ste 100

4.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8170 Adams Dr Ste 100 Ste 100
Hummelstown, PA 17036

900 Eisenhower Blvd Ste C

4.82 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-3:30 PM✓ Walk-in with Electronic Donor Pass
900 Eisenhower Blvd Ste C
Harrisburg, PA 17111

5 Capital Dr Ste 101

4.91 mi
🕑 Testing Hours: Mon–Sat: 8:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
5 Capital Dr Ste 101
Harrisburg, PA 17110

6301 Grayson Rd Spc 9

4.92 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
6301 Grayson Rd Spc 9
Harrisburg, PA 17111

645 N Mount Joy St

7.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
645 N Mount Joy St
Elizabethtown, PA 17022

4824 Londonderry Rd,

7.12 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
4824 Londonderry Rd,
Harrisburg, PA 17109

4200 Union Deposit Rd Ste G

7.13 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:30 PM✓ Walk-in with Electronic Donor Pass
4200 Union Deposit Rd Ste G
Harrisburg, PA 17111

1515 Bridge St

8.08 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
1515 Bridge St
New Cumberland, PA 17070

4813 Jonestown Rd Ste 104

8.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4813 Jonestown Rd Ste 104
Harrisburg, PA 17109

4813 Jonestown Rd Ste 107

8.29 mi
🕑 Testing Hours: Mon–Fri: 10:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
4813 Jonestown Rd Ste 107
Harrisburg, PA 17109

K. Palaiologoy 26

9.23 mi
🕑 Testing Hours: M-Th 9:00 AM-5:00 PM • F 9:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
K. Palaiologoy 26
N. Smyrni, GA 17121

418 Cloverleaf Rd

9.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
418 Cloverleaf Rd
Elizabethtown, PA 17022

3401 Hartzdale Dr,

10.45 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-5:00 PM • Sat: 7:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
3401 Hartzdale Dr,
Camp Hill, PA 17011

3314 Market St Ste 205

11.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3314 Market St Ste 205
Camp Hill, PA 17011

2021 Linglestown Rd,

11.45 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-3:20 PM • Sat: 7:00 AM-12:20 PM✓ Walk-in with Electronic Donor Pass
2021 Linglestown Rd,
Harrisburg, PA 17110

4910 Ritter Rd

11.92 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
4910 Ritter Rd
Mechanicsburg, PA 17055

6108 Carlisle Pike

14.17 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-3:30 PM • Sat: 7:30 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
6108 Carlisle Pike
Mechanicsburg, PA 17050

400 Loucks Rd

15.06 mi
🕑 Testing Hours: M-Sun: 8:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
400 Loucks Rd
York, PA 17404

520 Greenbriar Rd

15.09 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
520 Greenbriar Rd
York, PA 17404

970 Loucks Rd Unit D

15.28 mi
🕑 Testing Hours: M-Th 9:00 AM-5:00 PM • F 9:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
970 Loucks Rd Unit D
York, PA 17404

6481 Carlisle Pike

15.65 mi
🕑 Testing Hours: Mon–Fri: 10:00 AM-11:30 AM & 1:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
6481 Carlisle Pike
Mechanicsburg, PA 17050

2250 E Market St

15.83 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-6:00 PM • Sat:,Sun: 9:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
2250 E Market St
York, PA 17402

325 S Belmont St

16.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
325 S Belmont St
York, PA 17403

1748 6th Ave,

16.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1748 6th Ave,
York, PA 17403

York Crossings, 2189 York Crossing Drive

16.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
York Crossings, 2189 York Crossing Drive
York, PA 17408

11 E Market St Ste 104

16.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11 E Market St Ste 104
York, PA 17401

199 S 4th St At 399 Walnut St

17.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
199 S 4th St At 399 Walnut St
Lebanon, PA 17042

Rt 422 West,

17.86 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-11:30 AM & 12:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
Rt 422 West,
Lebanon, PA 17042

1010 W Crestview Dr

17.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1010 W Crestview Dr
Lebanon, PA 17042

2168 Cloverfield Dr

18.17 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-11:00 AM & 1:00 PM-3:00 PM✓ Walk-in with Electronic Donor Pass
2168 Cloverfield Dr
Lebanon, PA 17046

239 Pauline Dr (s York Plaza)

18.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
239 Pauline Dr (s York Plaza)
York, PA 17402

Employment, DOT, Court Ordered, Probation, Family

At our 31 testing centers in Middletown, Pennsylvania, Accredited Drug Testing delivers extensive drug and alcohol testing solutions. Catering to both DOT and non-DOT standards, we perform urine drug screening, breath alcohol checks, EtG alcohol assessments, and hair drug diagnostics for individuals, corporate clients, and legal purposes. In Middletown, PA, swift test results and SAMSA-certified lab analysis are available, with services offered the same day and most centers conveniently located close to you. We also provide Occupational Health Testing, Clinical Testing, and Background Verification.

Contact us at (800) 221-4291 or sign up online. Simply pick your required test and select a nearby center; testing services are open to individuals, staff, or third parties. Booking a test is quick and straightforward, with our scheduling team ready to assist or tests available for 24/7 online scheduling. Enjoy our simplified and intuitive process to set up drug testing near Middletown seamlessly.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Middletown, PA

1

Order Online or Call

Select your test & search nearby Middletown collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Middletown, PA Drug Testing

Drug Testing Services Available at Our Middletown, PA Labs:

At our Middletown drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Pennsylvania Drug Testing Laws & Compliance in Middletown

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Middletown, PA adhering strictly to 43 P.S. § 951 et seq., federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
43 P.S. § 951 et seq.
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Pennsylvania law supports employer zero-tolerance workplace drug policies, allowing pre-employment, safety-sensitive, and reasonable suspicion testing programs.

Under 43 P.S. § 951 et seq., employers in Middletown may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Pennsylvania), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Middletown, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Middletown and Pennsylvania.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Middletown, PA

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Middletown to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Middletown Neighborhoods Covered:

● Downtown Middletown ● Central Business District ● North Middletown Commercial Corridor ● South Middletown Industrial Park ● East Middletown Metro Area ● West Middletown Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Middletown Main Street Commercial District • Middletown Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Middletown? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Middletown

Royalton • Highspire • Goldsboro • Hummelstown • Valley Green • Falmouth • Oberlin • Bressler-enhaut-oberlin

Middletown, PA Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Middletown.

Test Type & Local Panel Detection Window Common Use Cases in Middletown Turnaround Time
5-Panel Urine Screen in Middletown →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Middletown →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Middletown →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Middletown →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Middletown →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Middletown →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Middletown, PA

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Middletown.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Middletown facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Middletown, PA

Need immediate walk-in service in Middletown? View our Walk-In Drug Testing Near Me in Middletown, PA facility guide or order an instant electronic donor pass online.

Middletown Drug Testing

Quickly find trusted local drug testing centers in Middletown, PA — fast, convenient, and reliable every time!

Middletown DOT Drug Testing

Quickly find a local DOT drug testing center in Middletown, PA — fast, reliable, convenient nationwide service!

Middletown DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Middletown, PA.

Middletown Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Middletown, PA.v

Middletown Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Middletown, PA locations—results you can trust, every time!

Middletown Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Middletown, PA.

Middletown Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Middletown, PA.

Middletown 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Middletown, PA employers and individuals nationwide.

Middletown 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Middletown, PA workplace and personal testing needs.

Middletown On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Middletown, PA workplace compliant.

Middletown DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Middletown, PA drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Pennsylvania, workplace drug testing is governed by statutory guidelines under 43 P.S. § 951 et seq.. Private employers generally operate under a Pennsylvania Medical Marijuana Act (35 P.S. § 10231.2103), permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Pennsylvania who implement a certified Drug-Free Workplace Program in compliance with 43 P.S. § 951 et seq. can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Pennsylvania state law compliance standards, state statutes prohibits discrimination against certified medical marijuana cardholders. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 31 certified collection sites in the Middletown area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Middletown, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Excellent service!

Excellent service! Trish went above and beyond to find me a testing site as close to my home as possible. Results were faster than expected, as well. Thanks for your help, Trish and Accredited!

Es Hache - 4/13/2025

Reception is kind and helpful

Reception is kind and helpful , everything quick and simple , best urgent care

Yusuf Narmuradov - 11/19/2024

Fast and efficient service

Fast and efficient service for employers wanting to do pre employment drug screening that meets DOT requirements!!

Mary Thomas - 4/5/2025


(800) 221-4291