✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Charlotte, NC

With 33 testing centers in the Charlotte area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

33 Drug-Testing Centers in Charlotte

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

1100 S Mint St Ste 115

0.74 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 01:00 PM - 03:00 PM
1100 S Mint St Ste 115
Charlotte, NC 28203

801 Baxter St

0.77 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 03:00 PM
801 Baxter St
Charlotte, NC 28202

1614 South Blvd

1.17 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 12:00 PM, 02:00 PM - 03:30 PM
1614 South Blvd
Charlotte, NC 28203

820 East Blvd Ste 818

1.50 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 02:30 PM
820 East Blvd Ste 818
Charlotte, NC 28203

1918 Randolph Rd Ste 175

1.57 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:00 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:00 AM, 01:00 PM - 03:00 PM
1918 Randolph Rd Ste 175
Charlotte, NC 28207

1928 Randolph Rd Ste 109

1.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1928 Randolph Rd Ste 109
Charlotte, NC 28207

2628 Statesville Ave

2.06 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 04:00 PM
2628 Statesville Ave
Charlotte, NC 28206

Remit Only: P.o. Box 601591

2.67 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:00 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 01:00 PM - 03:00 PM
Remit Only: P.o. Box 601591
Charlotte, NC 28260

3250 Wilkinson Blvd Unit I

2.87 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM✓ Walk-in with Electronic Donor Pass
3250 Wilkinson Blvd Unit I
Charlotte, NC 28208

4221 Tuckaseegee Rd

3.21 mi
🕑 Facility: Mon–Fri: 06:00 AM - 02:30 PM⚡ Drug Screens: Mon–Fri: 06:00 AM - 02:30 PM
4221 Tuckaseegee Rd
Charlotte, NC 28208

3541 Randolph Rd Ste 110,

3.23 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
3541 Randolph Rd Ste 110,
Charlotte, NC 28211

402 E Sugar Creek Rd

3.44 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 03:00 PM
402 E Sugar Creek Rd
Charlotte, NC 28213

4016 Wilkinson Blvd Ste C

3.70 mi
🕑 Facility: Mon–Fri: 06:00 AM - 02:00 PM✓ Walk-in with Electronic Donor Pass
4016 Wilkinson Blvd Ste C
Charlotte, NC 28208

231 S Sharon Amity Rd

4.33 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 03:00 PM
231 S Sharon Amity Rd
Charlotte, NC 28211

5410 N Tryon St

4.35 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 06:30 AM - 02:30 PM
5410 N Tryon St
Charlotte, NC 28213

5736 N Tryon St Ste 226-c

4.69 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM✓ Walk-in with Electronic Donor Pass
5736 N Tryon St Ste 226-c
Charlotte, NC 28213

4600 Holbrook Dr

4.91 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM (Lunch: 12:00 PM - 12:30 PM)✓ Walk-in with Electronic Donor Pass
4600 Holbrook Dr
Charlotte, NC 28212

2407 Derita Ave

5.38 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-8:00 PM • Sat: 7:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
2407 Derita Ave
Charlotte, NC 28269

6111 Rumple Rd

5.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6111 Rumple Rd
Charlotte, NC 28262

5717 Albemarle Rd

5.49 mi
🕑 Facility: Mon–Fri: 06:00 AM - 02:00 PM⚡ Drug Screens: Mon–Fri: 06:00 AM - 02:00 PM
5717 Albemarle Rd
Charlotte, NC 28212

6101 Idlewild Rd

5.56 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 02:30 PM
6101 Idlewild Rd
Charlotte, NC 28212

6407 Idlewild Rd Ste 211

5.83 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:00 PM✓ Walk-in with Electronic Donor Pass
6407 Idlewild Rd Ste 211
Charlotte, NC 28212

2533 Penninger Cir

6.68 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
2533 Penninger Cir
Charlotte, NC 28262

5031g W Wt Harris Blvd

7.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5031g W Wt Harris Blvd
Charlotte, NC 28269

8310 Medical Plaza Dr Ste G

7.51 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
8310 Medical Plaza Dr Ste G
Charlotte, NC 28262

8800 N Tryon St

7.77 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
8800 N Tryon St
Charlotte, NC 28262

2136 Ayrsley Town Blvd Ste B

8.04 mi
🕑 Testing Hours: M-Sun: 24 hrs✓ Walk-in with Electronic Donor Pass
2136 Ayrsley Town Blvd Ste B
Charlotte, NC 28273

10806 Reames Rd Ste I

8.22 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
10806 Reames Rd Ste I
Charlotte, NC 28269

8943 S Tryon St Unit K

8.22 mi
🕑 Testing Hours: M,W-F 9:00 AM-5:00 PM • T 9:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
8943 S Tryon St Unit K
Charlotte, NC 28273

9200 Harris Corners Pkwy Ste K

8.24 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
9200 Harris Corners Pkwy Ste K
Charlotte, NC 28269

8500 Andrew Carnegie Blvd

8.33 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
8500 Andrew Carnegie Blvd
Charlotte, NC 28262

7004 Smith Corners Blvd Ste A

8.40 mi
🕑 Testing Hours: M-Sun: 9:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
7004 Smith Corners Blvd Ste A
Charlotte, NC 28269

378 Crompton St

8.64 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:30 PM • Sat: 8:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
378 Crompton St
Charlotte, NC 28273

Employment, DOT, Court Ordered, Probation, Family

Accredited Drug Testing delivers a full spectrum of drug and alcohol testing services at our 33 testing facilities in Charlotte, North Carolina. We offer DOT and non-DOT urine tests, breathalyzer alcohol tests, EtG screenings, as well as hair follicle testing for individuals, companies, and legal purposes. In Charlotte, NC, we ensure prompt results and utilize SAMSA-certified labs. Same-day appointments are possible, and many facilities are conveniently situated close to homes or businesses. Additional offerings encompass Occupational Health Testing, Clinical Testing, and Background Checks.

Contact us at (800) 221-4291 or sign up online. Choose your test type and a convenient location nearby—testing is available whether for personal use, employment, or another party. Organizing a test is simple; reach out to our scheduling team or book online any time. Our efficient system makes it easy to set up a drug test near Charlotte without hassle.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Charlotte, NC

1

Order Online or Call

Select your test & search nearby Charlotte collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Charlotte, NC Drug Testing

Drug Testing Services Available at Our Charlotte, NC Labs:

At our Charlotte drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

North Carolina Drug Testing Laws & Compliance in Charlotte

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Charlotte, NC adhering strictly to N.C.G.S. § 95-230 et seq., federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
N.C.G.S. § 95-230 et seq.
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

North Carolina Controlled Substance Examination Regulation Act details approved sample collection, screening, and re-testing rights for job applicants and employees.

Under N.C.G.S. § 95-230 et seq., employers in Charlotte may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in North Carolina), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Charlotte, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Charlotte and North Carolina.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Charlotte, NC

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Charlotte to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Charlotte Neighborhoods Covered:

● Uptown ● South End ● NoDa (North Davidson) ● Plaza Midwood ● Dilworth ● Ballantyne ● University City ● Myers Park ● Elizabeth ● SouthPark

Major Employment & Commercial Hubs Served:

Uptown Banking & Financial Core • Ballantyne Corporate Park • SouthPark Commercial District • CLT Airport Logistics Corridor

Primary Charlotte ZIP Codes Served:

28202 28203 28204 28205 28209 28210 28277
Located anywhere in Charlotte? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Charlotte

West End • Derita • Paw Creek • Pineville • Matthews • Mount Holly • Woodland • Belmont

Charlotte, NC Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Charlotte.

Test Type & Local Panel Detection Window Common Use Cases in Charlotte Turnaround Time
5-Panel Urine Screen in Charlotte →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Charlotte →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Charlotte →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Charlotte →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Charlotte →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Charlotte →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Charlotte, NC

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Charlotte.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Charlotte facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Charlotte, NC

Need immediate walk-in service in Charlotte? View our Walk-In Drug Testing Near Me in Charlotte, NC facility guide or order an instant electronic donor pass online.

Charlotte Drug Testing

Quickly find trusted local drug testing centers in Charlotte, NC — fast, convenient, and reliable every time!

Charlotte DOT Drug Testing

Quickly find a local DOT drug testing center in Charlotte, NC — fast, reliable, convenient nationwide service!

Charlotte DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Charlotte, NC.

Charlotte Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Charlotte, NC.v

Charlotte Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Charlotte, NC locations—results you can trust, every time!

Charlotte Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Charlotte, NC.

Charlotte Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Charlotte, NC.

Charlotte 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Charlotte, NC employers and individuals nationwide.

Charlotte 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Charlotte, NC workplace and personal testing needs.

Charlotte On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Charlotte, NC workplace compliant.

Charlotte DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Charlotte, NC drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In North Carolina, workplace drug testing is governed by statutory guidelines under N.C.G.S. § 95-230 et seq.. Private employers generally operate under a Controlled Substance Examination Regulation Act, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in North Carolina who implement a certified Drug-Free Workplace Program in compliance with N.C.G.S. § 95-230 et seq. can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under North Carolina state law compliance standards, state statutes allows voluntary drug-free workplace policies and testing. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 33 certified collection sites in the Charlotte area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Charlotte, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Clear and Competent

I was so surprised with the quality of customer service I received today when I called Accredited Drug Testing. The representative was so clear and competent. She was able to reschedule an appointment at my preferred location. The representative whose name is Justice, was so polite and competent. She expedited the whole process. It was a relief to me.

Rachel Padian - 9/6/2024

Fantastic Experience

Fantastic experience from the beginning. Jocelyn was very helpful to me as someone who doesn’t have much experience in needing a test. They were very helpful and walked me through the entire process start to finish. Thank you ADT!

Riley Wilson - 2/18/2025

ADT was able to get me the best tests

Dealing with allegation’s of drug use that completely false is stressful, ADT was able to get me the best tests, going back the farthest in the same day with as little hassle as possible, I spoke with 1 person and wasn’t transferred once, and they took my payment, and got me my work order for a clinic as close as possible for the service I needed. 5*

Christopher Hansis - 12/19/2024


(800) 221-4291