✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Jacksonville, FL

With 37 testing centers in the Jacksonville area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

37 Drug-Testing Centers in Jacksonville

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

47 W Adams St

0.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
47 W Adams St
Jacksonville, FL 32202

300 W Adams St Ste 240

0.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 W Adams St Ste 240
Jacksonville, FL 32202

2545 Riverside Ave Ste 3

1.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2545 Riverside Ave Ste 3
Jacksonville, FL 32205

1325 San Marco Blvd Ste 301

1.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1325 San Marco Blvd Ste 301
Jacksonville, FL 32207

2570 Atlantic Blvd

2.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2570 Atlantic Blvd
Jacksonville, FL 32207

2063 Oak St

2.10 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-4:00 PM • Sat: 10:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
2063 Oak St
Jacksonville, FL 32204

5212 N Pearl St

2.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5212 N Pearl St
Jacksonville, FL 32208

5685 Norwood Ave

2.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5685 Norwood Ave
Jacksonville, FL 32208

1021 Cesery Blvd

3.51 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1021 Cesery Blvd
Jacksonville, FL 32211

3109 Spring Glen Rd Ste 302

3.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3109 Spring Glen Rd Ste 302
Jacksonville, FL 32207

4498 Hendricks Ave

3.99 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
4498 Hendricks Ave
Jacksonville, FL 32207

5960 Beach Blvd Ste 3

4.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5960 Beach Blvd Ste 3
Jacksonville, FL 32207

5960 Beach Blvd

4.21 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM (Lunch: 12:00 PM - 12:30 PM)✓ Walk-in with Electronic Donor Pass
5960 Beach Blvd
Jacksonville, FL 32207

837 Edgewood Ave N

4.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
837 Edgewood Ave N
Jacksonville, FL 32254

3901 University Blvd S Ste 203

4.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3901 University Blvd S Ste 203
Jacksonville, FL 32216

1718 Edgewood Ave N

4.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1718 Edgewood Ave N
Jacksonville, FL 32254

1610 Blanding Blvd

5.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1610 Blanding Blvd
Jacksonville, FL 32210

4933-1 University Blvd W

5.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4933-1 University Blvd W
Jacksonville, FL 32216

1912 Hamilton St Ste 103

5.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1912 Hamilton St Ste 103
Jacksonville, FL 32210

4616 San Juan Ave

5.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4616 San Juan Ave
Jacksonville, FL 32210

5964 Normandy Blvd

5.85 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
5964 Normandy Blvd
Jacksonville, FL 32205

6639 Southpoint Pkwy Ste 106

6.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6639 Southpoint Pkwy Ste 106
Jacksonville, FL 32216

5501 Roosevelt Blvd

6.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5501 Roosevelt Blvd
Jacksonville, FL 32244

4063 Salisbury Rd Ste 205

6.41 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4063 Salisbury Rd Ste 205
Jacksonville, FL 32216

7033 Commonwealth Ave Ste 8

6.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7033 Commonwealth Ave Ste 8
Jacksonville, FL 32220

4237 Salisbury Rd Ste 304

6.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4237 Salisbury Rd Ste 304
Jacksonville, FL 32216

6671 Hyde Grove Ave

6.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6671 Hyde Grove Ave
Jacksonville, FL 32210

1403 Dunn Ave Ste 26

6.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1403 Dunn Ave Ste 26
Jacksonville, FL 32218

1680 Dunn Ave Ste 39

6.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1680 Dunn Ave Ste 39
Jacksonville, FL 32218

1205 Monument Rd Ste 203

7.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1205 Monument Rd Ste 203
Jacksonville, FL 32225

4372 Southside Blvd, Ste 304

7.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4372 Southside Blvd, Ste 304
Jacksonville, FL 32216

231 Airline Way

7.41 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
231 Airline Way
Jacksonville, FL 32217

2032 Dunn Ave

7.48 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2032 Dunn Ave
Jacksonville, FL 32218

1584 Normandy Village Parkway Ste 32

7.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1584 Normandy Village Parkway Ste 32
Jacksonville, FL 32221

2262 Dunn Ave Ste 2

7.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2262 Dunn Ave Ste 2
Jacksonville, FL 32218

2255 Dunn Ave

7.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2255 Dunn Ave
Jacksonville, FL 32218

7933 Baymeadows Way Ste 3

7.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7933 Baymeadows Way Ste 3
Jacksonville, FL 32256

Employment, DOT, Court Ordered, Probation, Family

At our 37 testing centers in the Jacksonville, FL region, Accredited Drug Testing provides a wide array of drug and alcohol screening services. We cater to individuals, employers, and legal requirements with DOT and non-DOT urine drug tests, breath alcohol tests, EtG alcohol assessments, and hair drug screenings. Most testing centers are just a short distance from your home or office in Jacksonville, ensuring quick access to either rapid result evaluations or SAMSA certified lab analyses. We also offer Occupational Health Testing, Clinical Testing, and Background Checks.

To get started, contact us at (800) 221-4291 or register via our website. Choose the specific test and select a location nearby—ideal for personal use, for your workforce, or another person. Our scheduling service is convenient; reach out to our booking team or schedule an appointment online anytime. Thanks to our simplified process, coordinating drug testing near Jacksonville is seamless and efficient.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Jacksonville, FL

1

Order Online or Call

Select your test & search nearby Jacksonville collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Jacksonville, FL Drug Testing

Drug Testing Services Available at Our Jacksonville, FL Labs:

At our Jacksonville drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Florida Drug Testing Laws & Compliance in Jacksonville

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Jacksonville, FL adhering strictly to Fla. Stat. § 440.102, federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
Fla. Stat. § 440.102
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Florida Statute 440.102 establishes Drug-Free Workplace Program standards, providing premium discounts on worker compensation insurance for verified testing procedures.

Under Fla. Stat. § 440.102, employers in Jacksonville may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Florida), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Jacksonville, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Jacksonville and Florida.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Jacksonville, FL

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Jacksonville to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Jacksonville Neighborhoods Covered:

● Downtown Jacksonville ● San Marco ● Riverside ● Avondale ● Southside ● Mandarin ● Northside ● Jacksonville Beach ● Arlington ● Baymeadows

Major Employment & Commercial Hubs Served:

Southside Commercial Corridor • JAXPORT Maritime Logistics Hub • Cecil Commerce Center • Baymeadows Technology District

Primary Jacksonville ZIP Codes Served:

32202 32204 32207 32216 32224 32256 32258
Located anywhere in Jacksonville? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Jacksonville

Bellair-meadowbrook Terrace • Meadowbrook Terrace • Ratliff • Fruit Cove • Atlantic Beach • Neptune Beach • Lakeside • Nassau Village-ratliff

Jacksonville, FL Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Jacksonville.

Test Type & Local Panel Detection Window Common Use Cases in Jacksonville Turnaround Time
5-Panel Urine Screen in Jacksonville →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Jacksonville →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Jacksonville →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Jacksonville →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Jacksonville →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Jacksonville →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Jacksonville, FL

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Jacksonville.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Jacksonville facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Jacksonville, FL

Need immediate walk-in service in Jacksonville? View our Walk-In Drug Testing Near Me in Jacksonville, FL facility guide or order an instant electronic donor pass online.

Jacksonville Drug Testing

Quickly find trusted local drug testing centers in Jacksonville, FL — fast, convenient, and reliable every time!

Jacksonville DOT Drug Testing

Quickly find a local DOT drug testing center in Jacksonville, FL — fast, reliable, convenient nationwide service!

Jacksonville DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Jacksonville, FL.

Jacksonville Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Jacksonville, FL.v

Jacksonville Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Jacksonville, FL locations—results you can trust, every time!

Jacksonville Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Jacksonville, FL.

Jacksonville Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Jacksonville, FL.

Jacksonville 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Jacksonville, FL employers and individuals nationwide.

Jacksonville 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Jacksonville, FL workplace and personal testing needs.

Jacksonville On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Jacksonville, FL workplace compliant.

Jacksonville DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Jacksonville, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Florida, workplace drug testing is governed by statutory guidelines under Fla. Stat. § 440.102. Private employers generally operate under a Drug-Free Workplace Program under Fla. Stat. § 440.102, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Florida who implement a certified Drug-Free Workplace Program in compliance with Fla. Stat. § 440.102 can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Florida state law compliance standards, state statutes does not require accommodation of medical marijuana in workplace. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 37 certified collection sites in the Jacksonville area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Jacksonville, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

Excellant Performance

The visit here is always the best . The place is always really clean. The employees are super courteous, very polite, and professional. This is the only drug lab I like to go do my drug and alcohol test. I would like to tell them thank you so much for thier excellent performance and job

Eli Gonzalez - 1/4/2025

Accredited has been wonderful to work with!

Accredited has been wonderful to work with! My court ordered tests are on a very strange schedule but every time I call, they are quick to schedule me and are always able to find a location that works for where I’m at and when! Thank you for making this rather undesirable situation less stressful and efficient.

Madison Sanderford - 8/19/2024


(800) 221-4291