DOT Drug Testing - Jacksonville, TX

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 40 testing centers in the Jacksonville area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

40 DOT Drug-Testing Centers in Jacksonville

Minutes Away, Test Today

501 S Ragsdale St

0.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
501 S Ragsdale St
Jacksonville, TX 75766

555 S Jackson St

0.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
555 S Jackson St
Jacksonville, TX 75766

2026 S Jackson St

2.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2026 S Jackson St
Jacksonville, TX 75766

1325 N Dickinson Dr

12.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1325 N Dickinson Dr
Rusk, TX 75785

7924 S Broadway Ave

21.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7924 S Broadway Ave
Tyler, TX 75703

305 N Sycamore St 7

22.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
305 N Sycamore St 7
Palestine, TX 75801

4713 Troup Hwy

23.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4713 Troup Hwy
Tyler, TX 75703

1809 Capital Dr

23.31 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
1809 Capital Dr
Tyler, TX 75701

5040 Kinsey Dr Ste 500

23.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5040 Kinsey Dr Ste 500
Tyler, TX 75703

4520 S Broadway Ave

23.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4520 S Broadway Ave
Tyler, TX 75703

4290 Kinsey Dr Ste 200

23.54 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4290 Kinsey Dr Ste 200
Tyler, TX 75703

1819 Troup Hwy

24.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1819 Troup Hwy
Tyler, TX 75701

3110 Park Center Dr

24.50 mi
🕑 Testing Hours: M-Th 8:00 AM-5:00 PM • F 8:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
3110 Park Center Dr
Tyler, TX 75701

3180 Park Center Dr

24.59 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
3180 Park Center Dr
Tyler, TX 75701

2223 S Loop 256

25.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2223 S Loop 256
Palestine, TX 75801

1920 S Loop 256

25.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1920 S Loop 256
Palestine, TX 75801

700 Olympic Plaza Cir Ste 600

25.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
700 Olympic Plaza Cir Ste 600
Tyler, TX 75701

822 S Fleishel Avenue,

26.02 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-11:30 AM & 1:00 PM-4:00 PM✓ Walk-in with Electronic Donor Pass
822 S Fleishel Avenue,
Tyler, TX 75701

747 S Beckham Ave

26.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
747 S Beckham Ave
Tyler, TX 75701

649 S. Broadway Ave, Suite 1

26.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
649 S. Broadway Ave, Suite 1
Tyler, TX 75701

518 S Fleishel Ave

26.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
518 S Fleishel Ave
Tyler, TX 75702

323 S Fannin Ave

26.58 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
323 S Fannin Ave
Tyler, TX 75702

2507 W Oak St

27.28 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
2507 W Oak St
Palestine, TX 75801

3820 State Highway 64 W

27.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3820 State Highway 64 W
Tyler, TX 75704

2908 W Oak St

27.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2908 W Oak St
Palestine, TX 75801

300 Wilson St

30.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 Wilson St
Henderson, TX 75652

11937 Us Highway 271

32.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11937 Us Highway 271
Tyler, TX 75708

302 Little Ln

33.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
302 Little Ln
Kilgore, TX 75662

13239 Fm 782 N

33.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13239 Fm 782 N
Henderson, TX 75652

3203 S Main St

35.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3203 S Main St
Lindale, TX 75771

2000 S Palestine St

36.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2000 S Palestine St
Athens, TX 75751

203 Us Highway 175 W Ste 100

37.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
203 Us Highway 175 W Ste 100
Athens, TX 75751

1121 N Longview St

37.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1121 N Longview St
Kilgore, TX 75662

117 Medical Cir

38.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
117 Medical Cir
Athens, TX 75751

1505 Highway 19 South

38.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1505 Highway 19 South
Athens, TX 75751

5032 Justin St Ste 303

41.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5032 Justin St Ste 303
Nacogdoches, TX 75965

5021 Justin St Ste C

41.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5021 Justin St Ste C
Nacogdoches, TX 75965

4920 Ne Stallings Dr

42.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4920 Ne Stallings Dr
Nacogdoches, TX 75965

4909 North St

42.09 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
4909 North St
Nacogdoches, TX 75965

307 W Upshaw Ave

42.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
307 W Upshaw Ave
Gladewater, TX 75647

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 40 DOT drug testing locations to serve you in and around Jacksonville, TX.

Our Jacksonville, TX DOT drug testing centers, along with surrounding areas, offer comprehensive solutions such as DOT Drug Testing, Alcohol Testing, DOT Physicals, Random Pools, Consortium Enrollment, MIS, MVR, driver qualification reports, and additional DOT requirements per 49 CFR Part 40. These services are applicable for Employers, Single Operators, and all DOT modes like FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Conveniently, testing centers are close to your home or office, with same-day service often available.

Choose your preferred DOT service or test, select a nearby location, and streamline your process effortlessly—ideal for individuals, employees, or others. Arranging testing near Jacksonville is simple and user-friendly.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Jacksonville, Texas DOT Services

DOT Drug Testing & Services Available in Jacksonville, TX:

Located in Jacksonville, TX, our DOT services provide a comprehensive suite of testing options for both drug and alcohol detection. Designed to adhere to stringent industry standards, these services ensure compliance and safety in various operational sectors across Jacksonville, Texas.

DOT Services in Jacksonville, TX

DOT regulations are vital for ensuring employee safety in Jacksonville, TX. Employers must comply with drug and alcohol screening rules to maintain a safe workplace, protecting both employees and the public.

Under DOT regulations, Jacksonville, Texas employers must implement reasonable suspicion testing programs. This ensures testing is conducted fairly and employees’ rights are respected while safeguarding workspace integrity.

Random drug and alcohol testing is a core component of DOT compliance in Jacksonville, TX. This keeps employees alert and upholds safety standards essential for operations involving heavy machinery or public transport.

DOT regulations require employers in Jacksonville, Texas to maintain thorough documentation and reporting for screenings. This transparency fosters accountability and strengthens overall employee safety practices.

FMCSA Information

Jacksonville, TX mandates FMCSA-regulated employee testing to enhance transport safety. Employers must conduct regular drug and alcohol tests to maintain high operational safety standards.

FMCSA guidelines in Jacksonville, Texas require random drug testing, ensuring drivers’ adherence to safety rules and maintaining road safety integrity.

USCG Information

For maritime safety in Jacksonville, TX, USCG rules require drug and alcohol testing among seafarers, essential for marine operations’ security.

USCG regulations in Jacksonville, Texas mandate regular testing, focusing on maintaining safety across maritime industries.

FAA Information

Adherence to FAA testing rules in Jacksonville, TX is crucial for aviation safety. Employees undergo drug testing to maintain high standards of aviation operations.

FAA regulations require thorough drug testing in Jacksonville, Texas, safeguarding air travel safety and ensuring compliance with national safety standards.

FRA Information

The FRA in Jacksonville, TX enforces drug and alcohol testing for railroad safety. Compliance with these rules is crucial for preventing rail accidents and ensuring smooth operations.

In Jacksonville, Texas, FRA guidelines compel regular testing of rail workers, maintaining high safety standards and operational reliability.

FTA Information

Ensuring public transportation safety in Jacksonville, TX involves FTA-compliant drug and alcohol tests, necessary for maintaining a secure transit environment.

FTA regulations in Jacksonville, Texas enforce testing within public transit systems to uphold safety for commuters and transit employees alike.

PHMSA Information

In Jacksonville, TX, PHMSA rules ensure pipeline and hazardous material handling personnel comply with drug and alcohol testing requirements for safe operations.

PHMSA mandates testing in Jacksonville, Texas, critical for risk reduction and enhancing safety in pipeline and hazardous material transportation sectors.

Scheduling in Jacksonville, TX

Schedule your DOT drug or alcohol test in Jacksonville, TX by contacting our local team at (800) 221-4291. Alternatively, utilize our express online registration to select and register for your test before visiting the center.

Your entered zip code identifies the nearest Jacksonville, TX DOT testing center. A donor pass will be emailed containing center details and instructions. Bring this form or have it on your smartphone. Appointments are mostly unnecessary. Ensure the donor section is complete and payment is made upon registration.

Testing is conducted at SAMHSA-certified labs, and results are reviewed by our licensed Medical Review Officers (MRO.)

Besides offering DOT drug and alcohol testing in Jacksonville, TX, Accredited Drug Testing has centers across the surrounding areas.

Wherever you are, Accredited Drug Testing offers nationwide test scheduling anytime, anywhere!

Texas Dept. of Transportation Updates

DUI Statistics in Jacksonville, TX

The city of Jacksonville, TX, located in Cherokee County, experiences its share of alcohol-related traffic incidents. The Department of Transportation (DOT) classifies DUI accidents as critical given the damage they cause. In 2022, there were approximately X reported DUI-related accidents in Cherokee County. Awareness campaigns targeting DUI prevention have been initiated, emphasizing the risks and legal consequences in Texas (TX). Law enforcement collaborates with community organizations to reduce DUI incidents. The DOT supports these local efforts by providing data and resources to facilitate safer roadways in Jacksonville, TX. Texas continues to enforce stringent DUI laws to curb the crisis.

Drug-Involved Accidents in Jacksonville, TX

Jacksonville, TX, though a relatively small city, contends with numerous challenges, one of which includes drug-involved traffic accidents. Cherokee County records indicate a noticeable portion of traffic incidents involve substances other than alcohol. In recent reports, the number of drug-related accidents showed an increase of X%, highlighting an urgent need for intervention. The DOT, alongside Texas (TX) state authorities, is intensifying efforts on driver education concerning the dangers of driving under the influence of drugs. Local Jacksonville police have stepped up patrols and checkpoints to deter such behavior, striving for safer roads in line with DOT guidelines.

Marijuana-Related Accidents in Cherokee County, TX

With changing drug policies across the United States, marijuana-related accidents in areas like Jacksonville, TX, are under scrutiny. Cherokee County has recorded X cases where marijuana was a critical factor in traffic collisions. As this trend continues, the Department of Transportation (DOT) in Texas stresses the importance of patient-centered education about the effects of marijuana on driving abilities. Public awareness campaigns are essential in Jacksonville and broader TX regions to mitigate this risk. By increasing roadside drug testing and equipping officers with advanced detection tools, the DOT and local authorities aim to reduce these incidents and promote safer driving practices throughout Cherokee County, TX.

Jacksonville Drug Testing

Quickly find trusted local drug testing centers in Jacksonville, TX — fast, convenient, and reliable every time!

Jacksonville DOT Drug Testing

Quickly find a local DOT drug testing center in Jacksonville, TX — fast, reliable, convenient nationwide service!

Jacksonville DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Jacksonville, TX.

Jacksonville Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Jacksonville, TX.v

Jacksonville Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Jacksonville, TX locations—results you can trust, every time!

Jacksonville Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Jacksonville, TX.

Jacksonville Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Jacksonville, TX.

Jacksonville 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Jacksonville, TX employers and individuals nationwide.

Jacksonville 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Jacksonville, TX workplace and personal testing needs.

Jacksonville On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Jacksonville, TX workplace compliant.

Jacksonville DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Jacksonville, TX drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

Super Helpful

Detricia was super helpful in making sure that the drug test is sent my worker for was the right one I needed for my company to be compliant with my customers drug test requirements.

Kevin Howell - 12/19/2024

Very professional and very quick

Very professional and very quick service to schedule appointments! I am pleased with the services and would definitely recommend using them for any drug testing needs!

Carrie Feerick - 4/19/2025

Quick, knowledgeable and courteous

Quick, knowledgeable and courteous Janelle worked diligently to support me. I am recommending their services to anyone looking.

Greensboro Joseph - 11/19/2024


(800) 221-4291