DOT Drug Testing - Jefferson, NC

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 40 testing centers in the Jefferson area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

40 DOT Drug-Testing Centers in Jefferson

Minutes Away, Test Today

151 Medical Park Dr

0.68 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
151 Medical Park Dr
Jefferson, NC 28640

200 Hospital Ave Send Split Kits Only

4.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
200 Hospital Ave Send Split Kits Only
Jefferson, NC 28640

6436 Troutdale Hwy

16.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6436 Troutdale Hwy
Troutdale, VA 24378

1901 Sandy Rd

18.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1901 Sandy Rd
Mountain City, TN 37683

381 Deerfield Rd

18.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
381 Deerfield Rd
Boone, NC 28607

336 Deerfield Rd

18.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
336 Deerfield Rd
Boone, NC 28607

20471 Azen Rd

19.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
20471 Azen Rd
Damascus, VA 24236

67 High Country Ln

19.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
67 High Country Ln
Troutdale, VA 24378

233 Doctors St

20.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
233 Doctors St
Sparta, NC 28675

306 Shady Ave

22.92 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-11:00 AM & 1:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
306 Shady Ave
Damascus, VA 24236

1903 Addison Ave

24.25 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-7:30 PM • Sat: 9:00 AM-4:30 PM • Sun: 10:00 AM-5:30 PM✓ Walk-in with Electronic Donor Pass
1903 Addison Ave
Wilkesboro, NC 28697

1900 W Park Dr

24.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1900 W Park Dr
North Wilkesboro, NC 28659

37 Boone Trl

24.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
37 Boone Trl
North Wilkesboro, NC 28659

1916 W Park Dr

24.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1916 W Park Dr
North Wilkesboro, NC 28659

1918 W Park Dr

25.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1918 W Park Dr
North Wilkesboro, NC 28659

702 13th St

25.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
702 13th St
North Wilkesboro, NC 28659

178 Highway 105 Ext Ste 101

25.18 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
178 Highway 105 Ext Ste 101
Boone, NC 28607

1201 School Street Ste A

25.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1201 School Street Ste A
Wilkesboro, NC 28697

207 10th St

25.43 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
207 10th St
North Wilkesboro, NC 28659

1201 School St Ste G

25.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1201 School St Ste G
Wilkesboro, NC 28697

308 E Main St

25.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
308 E Main St
Wilkesboro, NC 28697

245 Medical Park Dr Smyth County Community Hospital

28.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
245 Medical Park Dr Smyth County Community Hospital
Marion, VA 24354

134 E Lee Hwy

28.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
134 E Lee Hwy
Chilhowie, VA 24319

403 Chilhowie St

28.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
403 Chilhowie St
Chilhowie, VA 24319

1046 Terrace Dr

29.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1046 Terrace Dr
Marion, VA 24354

636 S Monte Vista Dr

30.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
636 S Monte Vista Dr
Glade Spring, VA 24340

13121 Kincannon Rd

30.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13121 Kincannon Rd
Glade Spring, VA 24340

24530 Falcon Place Blvd Ste 100

31.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
24530 Falcon Place Blvd Ste 100
Abingdon, VA 24211

16000 Johnston Memorial Dr

31.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
16000 Johnston Memorial Dr
Abingdon, VA 24211

609 E Main St

33.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
609 E Main St
Abingdon, VA 24210

470 E Main St

34.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
470 E Main St
Abingdon, VA 24210

200 Hospital Dr

34.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
200 Hospital Dr
Galax, VA 24333

199 Hospital Dr

35.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
199 Hospital Dr
Galax, VA 24333

603 Campus Dr Ste 100

35.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
603 Campus Dr Ste 100
Abingdon, VA 24210

308 Mulberry St Sw

35.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
308 Mulberry St Sw
Lenoir, NC 28645

321 Mulberry St Sw

35.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
321 Mulberry St Sw
Lenoir, NC 28645

381 W Main St

35.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
381 W Main St
Saltville, VA 24370

1550 N Bridge St

35.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1550 N Bridge St
Elkin, NC 28621

942 Johnson Ridge Road, Ste U

36.38 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:30 AM, 01:00 PM - 04:30 PM
942 Johnson Ridge Road, Ste U
Elkin, NC 28621

400 Johnson Ridge Medical Park

36.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
400 Johnson Ridge Medical Park
Elkin, NC 28621

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 40 DOT drug testing locations to serve you in and around Jefferson, NC.

In Jefferson, NC, our local DOT drug testing centers deliver a range of services such as DOT Drug and Alcohol Testing, Physicals, Random pool, Consortium enrollment, MIS and MVR reports, as well as driver qualification reports. Catering to Employers, Single Operators, and all DOT modes under 49 CFR Part 40, including FMCSA, FTA, FAA, USCG, FRA, and PHMSA, our locations are easily accessible. Often, same-day services are available near your home or office.

Choose the required DOT service or drug/alcohol test(s) and find a convenient location. Our streamlined process ensures easy arrangement of DOT drug testing near Jefferson for individuals, employees, or others.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Jefferson, North Carolina DOT Services

DOT Drug Testing & Services Available in Jefferson, NC:

In Jefferson, NC, Accredited Drug Testing offers a variety of DOT services, including drug and alcohol testing. Our streamlined process ensures ease and convenience for all testing needs in Jefferson, North Carolina.

DOT Services in Jefferson, NC

DOT regulations mandate strict drug and alcohol screenings to promote workplace safety. In Jefferson, NC, companies must implement random screenings to comply with federal laws and regulations.

Employers in Jefferson, North Carolina, under DOT regulations, must maintain records of employee screenings and ensure their compliance with drug-free workplace standards mandated by DOT rules.

Random drug testing is crucial in Jefferson, NC, to maintain safety standards as stipulated under DOT regulations. This helps in identifying and deterring substance abuse among employees.

DOT guidelines in Jefferson, North Carolina, emphasize employee education programs about drug and alcohol prevention, ensuring everyone's safety and adherence to federal mandates.

In Jefferson, NC, adhering to DOT drug and alcohol rules is paramount for public safety, necessitating routine screenings and compliance with comprehensive regulatory statutes.

FMCSA Information

FMCSA enforces drug testing in Jefferson, NC, to keep commercial drivers sober. Screenings help maintain high safety standards for transport operators.

Strict FMCSA rules in Jefferson, North Carolina require regular testing for commercial drivers to prevent substance impairments during operations.

USCG Information

USCG testing rules in Jefferson, NC aim to preserve safety on the waters by implementing drug tests for maritime operators.

In Jefferson, North Carolina, the USCG mandates drug testing for maritime personnel, ensuring secure and drug-free navigation.

FAA Information

FAA's regulations in Jefferson, NC, require aviation employees to undergo routine tests to prevent substance abuse in safety-critical roles.

In Jefferson, North Carolina, FAA ensures aviation safety through mandatory drug tests, supporting a secure flying environment.

FRA Information

The FRA mandates drug testing in Jefferson, NC, to ensure rail safety by preventing drug impairments during operations.

FRA rules in Jefferson, North Carolina demand frequent screenings to uphold rail safety standards by deterring drug use.

FTA Information

FTA's policies in Jefferson, NC enforce drug testing for public transport operators to ensure passenger safety.

Regular testing under FTA rules in Jefferson, North Carolina keeps public transportation operators compliant and passengers secure.

PHMSA Information

PHMSA mandates in Jefferson, NC focus on safety by enforcing drug screens for pipeline workers, maintaining hazard-free environments.

In Jefferson, North Carolina, PHMSA's drug testing rules ensure pipeline safety by regularly screening safety-sensitive employees.

Scheduling in Jefferson, NC

To arrange a DOT drug or alcohol test in Jefferson, NC, contact our local scheduling team at (800) 221-4291 or register online using our express system. Choose your test and complete the Donor Information/Registration Section to ensure a smooth testing experience.

Enter your zip code to locate a nearby DOT testing facility in Jefferson, North Carolina. A donor pass with testing center details will be sent to your email. Present this form at the center; no appointment is needed. Ensure registration completion and test payment beforehand.

Our SAMHSA-certified laboratories ensure all results are verified by our in-house Medical Review Officers (MRO) who are licensed physicians.

Besides our Jefferson, NC location, Accredited Drug Testing offers comprehensive services throughout the metro and neighboring areas.

Accredited Drug Testing's extensive network, covering all cities nationwide, ensures testing availability anytime and anywhere, even during travels or vacations.

North Carolina Dept. of Transportation Updates

DUI Statistics in Jefferson, NC

In Jefferson, North Carolina (NC), and the surrounding Ashe County, the Department of Transportation (DOT) plays a significant role in managing and analyzing DUI-related incidents. According to the most recent data available, DUI arrests have shown a fluctuation over the past few years. The state of North Carolina reports that Ashe County, including Jefferson, experiences a higher rate of DUI incidents particularly during holiday seasons and major community events. These figures are critical for local law enforcement and DOT officials, who utilize this information to strategize enforcement and prevention measures. The collaboration between North Carolina DOT and local community organizations is crucial to educate the public on the dangers of driving under the influence, and to implement sobriety checkpoints and public awareness campaigns effectively.

Drug-Involved Accidents in Jefferson, NC

Jefferson, NC, and Ashe County, have seen a concerning number of drug-involved accidents over recent years. The North Carolina Department of Transportation (NC DOT) consistently monitors these incidents, noting that a significant percentage involve prescription drugs and illicit substances. With the rise of opioid use across the state, NC DOT and local authorities are prioritizing efforts to address this critical issue. In Ashe County, comprehensive data collection is underway to understand the extent of drug impairment in vehicular accidents. The goal is to use this information to shape policy and enhance road safety measures, which include increased law enforcement training and drug awareness programs tailored to the needs of Jefferson, NC residents, mitigating risks on the roadways.

Marijuana-Related Accidents in Jefferson, NC

In Jefferson, North Carolina, marijuana-related accidents have become a focal point for the Department of Transportation (DOT) and law enforcement agencies. As marijuana legislation evolves, its impact on road safety in Ashe County is a significant concern. The NC DOT has observed a correlation between lenient marijuana laws and the frequency of marijuana-related traffic incidents. Data suggests that while the total number of road accidents may not have risen sharply, the proportion involving marijuana has grown. This has prompted NC DOT and local authorities to increase educational outreach regarding the effects of marijuana on driving abilities. By providing Jefferson and Ashe County residents with access to information on the impairing effects of marijuana, the DOT aims to promote safer driving habits and to reduce marijuana-impaired driving incidents.

Jefferson Drug Testing

Quickly find trusted local drug testing centers in Jefferson, NC — fast, convenient, and reliable every time!

Jefferson DOT Drug Testing

Quickly find a local DOT drug testing center in Jefferson, NC — fast, reliable, convenient nationwide service!

Jefferson DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Jefferson, NC.

Jefferson Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Jefferson, NC.v

Jefferson Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Jefferson, NC locations—results you can trust, every time!

Jefferson Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Jefferson, NC.

Jefferson Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Jefferson, NC.

Jefferson 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Jefferson, NC employers and individuals nationwide.

Jefferson 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Jefferson, NC workplace and personal testing needs.

Jefferson On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Jefferson, NC workplace compliant.

Jefferson DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Jefferson, NC drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291