DOT Drug Testing - Durham, NC

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 37 testing centers in the Durham area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

37 DOT Drug-Testing Centers in Durham

Minutes Away, Test Today

2609 N Duke St Ste 301

2.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2609 N Duke St Ste 301
Durham, NC 27704

2609 N Duke St Ste 900

2.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2609 N Duke St Ste 900
Durham, NC 27704

3505 N Roxboro St

2.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3505 N Roxboro St
Durham, NC 27704

3209 Guess Rd Ste 201

3.36 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3209 Guess Rd Ste 201
Durham, NC 27705

3414 N Duke St Ste 400

3.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3414 N Duke St Ste 400
Durham, NC 27704

101 Medical Park Dr

3.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 Medical Park Dr
Mebane, NC 27302

3901 N Roxboro St Po Box 30428

5.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3901 N Roxboro St Po Box 30428
Durham, NC 27704

2945 South Miami Blvd #132

5.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2945 South Miami Blvd #132
Durham, NC 27703

7010 Nc Highway 751

6.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7010 Nc Highway 751
Durham, NC 27707

118 Old Durham Rd Ste B

6.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
118 Old Durham Rd Ste B
Chapel Hill, NC 27517

5607 Nc Highway 55 Ste 201

6.98 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
5607 Nc Highway 55 Ste 201
Durham, NC 27713

2238 Nelson Hwy Ste 500

7.79 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2238 Nelson Hwy Ste 500
Chapel Hill, NC 27517

1005 Slater Rd Ste 101

8.64 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
1005 Slater Rd Ste 101
Durham, NC 27703

7841 Alexander Promenade Pl Ste 120

8.67 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
7841 Alexander Promenade Pl Ste 120
Raleigh, NC 27617

1407 E Franklin St

8.81 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
1407 E Franklin St
Chapel Hill, NC 27514

4104 Surles Ct Ste 11

9.11 mi
🕑 Testing Hours: M-Th 9:00 AM-2:00 PM • F 9:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
4104 Surles Ct Ste 11
Durham, NC 27703

101 Manning Dr

10.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 Manning Dr
Chapel Hill, NC 27514

405 Meadowland Dr

11.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
405 Meadowland Dr
Hillsborough, NC 27278

8312 Creedmont Road

11.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8312 Creedmont Road
Raleigh, NC 27613

3721 Lynn Rd

13.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3721 Lynn Rd
Raleigh, NC 27613

1614 Nc Highway 56

14.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1614 Nc Highway 56
Creedmoor, NC 27522

1614 Nc Hwy 56

14.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1614 Nc Hwy 56
Creedmoor, NC 27522

100 Cornerstone Dr

14.35 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-7:30 PM • Sat: 9:00 AM-4:30 PM • Sun: 12:00 PM-4:30 PM✓ Walk-in with Electronic Donor Pass
100 Cornerstone Dr
Cary, NC 27519

6010 Glenwood Avenue

14.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6010 Glenwood Avenue
Raleigh, NC 27612

6500 Creedmoor Rd

14.84 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-7:30 PM • Sat: 9:00 AM-4:30 PM • Sun: 10:00 AM-5:30 PM✓ Walk-in with Electronic Donor Pass
6500 Creedmoor Rd
Raleigh, NC 27613

8300 Health Park, Sutie 223

15.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8300 Health Park, Sutie 223
Raleigh, NC 27615

101 Maynard Crossing Ct

15.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 Maynard Crossing Ct
Cary, NC 27513

3850 Ed Drive, Suite 125

16.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3850 Ed Drive, Suite 125
Raleigh, NC 27612

2406 Blue Ridge Rd Ste 190

16.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2406 Blue Ridge Rd Ste 190
Raleigh, NC 27607

1110 Kildaire Farm Rd

16.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1110 Kildaire Farm Rd
Cary, NC 27511

2021 Creekside Landing Dr Ste 110

17.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2021 Creekside Landing Dr Ste 110
Apex, NC 27502

5650 Six Forks Rd Ste 202

17.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5650 Six Forks Rd Ste 202
Raleigh, NC 27609

1515 Sw Cary Pkwy Ste 120

17.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1515 Sw Cary Pkwy Ste 120
Cary, NC 27511

1021 W Williams St Ste 102

17.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1021 W Williams St Ste 102
Apex, NC 27502

103 Baines Court,

17.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
103 Baines Court,
Cary, NC 27511

1911 Falls Valley Dr Ste 100

17.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1911 Falls Valley Dr Ste 100
Raleigh, NC 27615

10880 Durant Road, Suite 215

18.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10880 Durant Road, Suite 215
Raleigh, NC 27614

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 37 DOT drug testing locations to serve you in and around Durham, NC.

Our DOT testing sites across Durham, NC offer comprehensive services including DOT Drug and Alcohol Testing, Physicals, Consortium enrollment, and reports like MIS and MVR. Available for employers, single operators, or any DOT modes under 49 CFR Part 40 compliance, we cater to FMCSA, FTA, FAA, USCG, FRA, and PHMSA requirements. Conveniently located near homes or offices, most centers offer same-day service.

Select your DOT service or preferred drug/alcohol test(s) and find a nearby site. Testing is available for individuals, employees, or others; our straightforward system ensures arranging testing near Durham is quick and simple.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Durham, North Carolina DOT Services

DOT Drug Testing & Services Available in Durham, NC:

In Durham, NC, Accredited Drug Testing excels in delivering comprehensive DOT services including drug and alcohol screenings, physicals, and safety compliance for businesses, ensuring a secure work environment.

DOT Services in Durham, NC

Ensuring employee safety, DOT regulations demand stringent drug and alcohol screenings. In Durham, NC, compliance with these standards promotes a secure working environment for all.

DOT mandates rigorous testing protocols as part of its safety initiative for transportation employees in Durham, North Carolina. Accredited Drug Testing aligns its services to meet these critical regulatory requirements.

With DOT's comprehensive guidelines, Accredited Drug Testing in Durham, NC supports employers in maintaining a drug-free workplace, ensuring safety and compliance across different DOT modes.

Accredited Drug Testing's services ensure compliance with DOT's 49 CFR Part 40 in Durham, North Carolina, safeguarding both company interests and employee well-being through effective testing measures.

FMCSA Information

In Durham, NC, the FMCSA enforces strict drug and alcohol testing rules for commercial drivers, ensuring road safety and regulatory compliance.

Accredited Drug Testing in Durham, NC adheres to FMCSA's regulations, providing essential screening services for the transportation sector.

USCG Information

The USCG enforces drug and alcohol testing in Durham, NC, prioritizing maritime safety through diligent compliance efforts.

Accredited Drug Testing supports USCG mandates in Durham, North Carolina, ensuring a safe maritime work environment through effective testing protocols.

FAA Information

Durham, NC adheres to the FAA's strict testing guidelines to ensure aviation safety through comprehensive drug and alcohol compliance.

At Accredited Drug Testing, FAA testing in Durham, North Carolina is conducted to uphold safety and operational standards in aviation.

FRA Information

FRA regulations dictate drug and alcohol testing in Durham, NC, enhancing rail safety through stringent compliance measures.

Accredited Drug Testing supports FRA's safety goals in Durham, North Carolina by providing essential testing services for rail workers.

FTA Information

FTA drug and alcohol testing in Durham, NC ensures public transportation safety through stringent compliance, protecting commuters and staff.

Accredited Drug Testing in Durham, North Carolina aligns with FTA guidelines, promoting safe public transit operations.

PHMSA Information

PHMSA's drug and alcohol testing regulations in Durham, NC focus on pipeline industry safety, promoting a secure workforce through rigorous testing.

Accredited Drug Testing offers PHMSA-compliant services in Durham, North Carolina, ensuring safety in the pipeline sector.

Scheduling in Durham, NC

For DOT drug or alcohol testing appointments in Durham, NC, reach our scheduling team at (800) 221-4291 or register online. Use our express system to select the test and complete the required registration section before heading to the testing site.

Enter your zip code to pinpoint the nearest DOT testing center in Durham, North Carolina. Post-registration, you’ll get a donor pass with the center's address, hours, and instructions via email. Bring this pass to the center; no appointment needed, but complete donor info and payment during registration.

Testing is conducted in SAMHSA-certified labs, with all results reviewed by our in-house Medical Review Officers (MRO).

Beyond Durham, Accredited Drug Testing offers services at numerous other sites across the metro area and nearby cities.

Traveling? Don't worry—our extensive nationwide network ensures access to testing centers everywhere, anytime!

North Carolina Dept. of Transportation Updates

Durham, NC DUI Statistics

In recent years, Durham, North Carolina, has taken various initiatives to address DUI-related incidents. Reports suggest that within Durham County, the number of DUI arrests underscores ongoing concerns related to road safety. Law enforcement agencies have implemented stricter enforcement policies and increased patrols, particularly during weekends and holidays when DUI incidents are more prevalent. Initiatives such as public education campaigns aim to reduce the frequency of these occurrences. With North Carolina enforcing stringent DUI penalties, Durham continues to focus on spreading awareness about the risks of driving under the influence, striving to lower the overall DUI rates.

Drug-Involved Accidents in Durham, NC

Durham, NC, is actively addressing the challenge of drug-involved accidents on its roads. Recent data from Durham County indicate a troubling number of vehicular accidents where drug impairment was a factor. Law enforcement agencies are increasingly utilizing drug recognition experts to identify drivers under the influence of substances other than alcohol. North Carolina's Department of Transportation collaborates with local authorities to incorporate modern technologies and methodologies to combat this issue. Awareness campaigns and community workshops in and around Durham aim to educate the public on the dangers of drug-impaired driving, hoping to mitigate this persistent problem.

Marijuana-Related Accidents in Durham, NC

With evolving legislation around marijuana use, Durham, North Carolina, has seen a growing focus on marijuana-related road accidents. In Durham County, law enforcement encounters challenges in precisely measuring impairment due to marijuana. As North Carolina continues to navigate its legal stance on marijuana, proactive measures are taken to monitor its impact on road safety. The local government has intensified efforts to disseminate information about the potential risks of driving after marijuana consumption. Additionally, Durham's transportation department is looking into advanced testing methods to better assess impairment levels, emphasizing the need for updated training for officers to effectively address marijuana-related driving offenses.

Durham Drug Testing

Quickly find trusted local drug testing centers in Durham, NC — fast, convenient, and reliable every time!

Durham DOT Drug Testing

Quickly find a local DOT drug testing center in Durham, NC — fast, reliable, convenient nationwide service!

Durham DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Durham, NC.

Durham Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Durham, NC.v

Durham Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Durham, NC locations—results you can trust, every time!

Durham Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Durham, NC.

Durham Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Durham, NC.

Durham 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Durham, NC employers and individuals nationwide.

Durham 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Durham, NC workplace and personal testing needs.

Durham On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Durham, NC workplace compliant.

Durham DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Durham, NC drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291