DOT Drug Testing - Louisville, KY

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 29 testing centers in the Louisville area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

29 DOT Drug-Testing Centers in Louisville

Minutes Away, Test Today

1 Arena Plaza

0.34 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
1 Arena Plaza
Louisville, KY 40202

200 Abraham Flexner Way Emergency Dept

0.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
200 Abraham Flexner Way Emergency Dept
Louisville, KY 40202

645 S Roy Wilkins Ave

0.54 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-2:30 PM✓ Walk-in with Electronic Donor Pass
645 S Roy Wilkins Ave
Louisville, KY 40203

901 W Broadway

0.64 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
901 W Broadway
Louisville, KY 40203

200 Abraham Flexner Way

0.67 mi
🕑 Testing Hours: Mon–Fri: 10:00 AM-11:30 AM & 12:00 PM-1:00 PM • Sat: 8:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
200 Abraham Flexner Way
Louisville, KY 40202

610 S Floyd St Ste 100

0.71 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
610 S Floyd St Ste 100
Louisville, KY 40202

310 E Broadway

0.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
310 E Broadway
Louisville, KY 40202

1220 Missouri Ave

2.13 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 02:00 PM - 04:00 PM
1220 Missouri Ave
Jeffersonville, IN 47130

1227 Goss Ave

2.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1227 Goss Ave
Louisville, KY 40217

Department

2.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Department
Louisville, KY 40289

1169 Eastern Pkwy Ste 2343,

2.68 mi
🕑 Facility: Mon–Fri: 08:30 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 10:00 AM - 04:30 PM
1169 Eastern Pkwy Ste 2343,
Louisville, KY 40217

1169 Eastern Pkwy Ste G10

2.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1169 Eastern Pkwy Ste G10
Louisville, KY 40217

1602 E 10th St

3.20 mi
🕑 Facility: Mon–Fri: 07:00 AM - 08:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 05:00 PM
1602 E 10th St
Jeffersonville, IN 47130

2307 Bardstown Rd

4.29 mi
🕑 Testing Hours: Mon–Fri: 11:00 AM-2:00 PM • Sat: 8:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
2307 Bardstown Rd
Louisville, KY 40205

2470 Bardstown Rd Ste B

4.51 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-2:00 PM • Sat: 8:30 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
2470 Bardstown Rd Ste B
Louisville, KY 40205

1615 Blackiston View Dr

4.54 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 03:30 PM
1615 Blackiston View Dr
Clarksville, IN 47129

1850 Bluegrass Ave Emergency Dept

4.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Bluegrass Ave Emergency Dept
Louisville, KY 40215

2051 Clevidence Blvd Ste A

5.20 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:30 AM, 01:00 PM - 03:30 PM
2051 Clevidence Blvd Ste A
Clarksville, IN 47129

2201 Greentree N

5.33 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:00 AM, 12:30 PM - 04:00 PM
2201 Greentree N
Clarksville, IN 47129

1941 Bishop Ln Ste 205

5.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1941 Bishop Ln Ste 205
Louisville, KY 40218

3118 E 10th St

5.53 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 11:30 AM, 12:30 PM - 02:30 PM
3118 E 10th St
Jeffersonville, IN 47130

228 Breckenridge Ln 5

5.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
228 Breckenridge Ln 5
Louisville, KY 40207

3920 Dutchmans Pkwy Emergency Dept

5.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3920 Dutchmans Pkwy Emergency Dept
Louisville, KY 40207

3430 Newburg Rd Ste 106

5.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3430 Newburg Rd Ste 106
Louisville, KY 40218

4420 Dixie Hwy Ste 112

5.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4420 Dixie Hwy Ste 112
Louisville, KY 40216

5601 S 3rd St Basement

5.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5601 S 3rd St Basement
Louisville, KY 40214

3430 Newburg Rd Ste 110

5.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3430 Newburg Rd Ste 110
Louisville, KY 40218

825 University Woods Dr Ste 12

6.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
825 University Woods Dr Ste 12
New Albany, IN 47150

3605 Northgate Ct Ste 110

6.18 mi
🕑 Facility: Mon–Fri: 08:00 AM - 08:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 05:00 PM
3605 Northgate Ct Ste 110
New Albany, IN 47150

Serving All DOT modes
DOT Drug Testing, DOT Consortium,
FMCSA Clearinghouse, Physicals

We have 29 DOT drug testing locations to serve you in and around Louisville, KY.

Accredited Drug Testing provides a wide array of DOT services including drug, alcohol testing, physicals, and more. Our centers are conveniently located across Louisville, KY, ensuring quick access for employees, single operators, and all DOT modes needing compliance with 49 CFR Part 40, including FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Same-day service for testing is often available.

Choose from various DOT services or drug/alcohol tests, and easily arrange them at a conveniently located center for yourself or employees. Our process for scheduling DOT drug tests near Louisville is designed to be swift and simple.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Louisville, Kentucky DOT Services

DOT Drug Testing & Services Available in Louisville, KY:

In Louisville, KY, Accredited Drug Testing facilitates a variety of DOT services including mandatory drug and alcohol testing, complying with federal safety standards to ensure the highest level of employee safety.

DOT Services in Louisville, KY

DOT regulations necessitate rigorous drug and alcohol screening for employees to ensure safety, a critical factor in Louisville, KY. Compliance with these regulations promotes a safer work environment.

In Louisville, Kentucky, DOT rules on drug testing help prevent accidents by ensuring all employees operate without substance influences, prioritizing safety in all operations.

The DOT requires regular intervals of drug screening in Louisville, KY, ensuring protocols are followed to maintain high safety standards for employees and public alike.

At Accredited Drug Testing in Louisville, KY, our adherence to DOT regulations means we provide accurate and timely drug screenings to meet safety expectations consistently.

FMCSA Information

The FMCSA sets stringent drug and alcohol testing rules to ensure that commercial drivers in Louisville, KY, remain compliant and safe on the roads.

Adhering to FMCSA guidelines, Accredited Drug Testing in Louisville, KY, ensures drivers meet federal safety requirements.

USCG Information

USCG drug testing strategies ensure that maritime workers across Louisville, KY, maintain optimal safety just like on any other waters.

In Louisville, Kentucky, compliance with USCG testing protocols is integral to secure maritime operations.

FAA Information

FAA regulations in Louisville, KY, require drug testing for aviation personnel to maintain high safety standards within the air travel sector.

Accredited Drug Testing ensures compliance with FAA drug testing measures in Louisville, Kentucky, for flight safety assurances.

FRA Information

FRA drug and alcohol testing rules apply to rail workers in Louisville, KY, maintaining secure rail operations free from substance influence.

Accredited Drug Testing adheres to FRA requirements, ensuring top tier rail safety in Louisville, KY.

FTA Information

The FTA mandates drug testing to maintain public transit safety in Louisville, Kentucky, safeguarding passengers and workers alike.

Our Louisville, KY, centers align with FTA guidelines, promoting secure and substance-free transportation services.

PHMSA Information

PHMSA mandates comprehensive drug testing for pipeline workers in Louisville, Kentucky, promoting operational safety and compliance across the industry.

In Louisville, KY, our services meet PHMSA testing rules, essential for maintaining safe energy transport pipelines.

Scheduling in Louisville, KY

Call (800) 221-4291 to arrange DOT drug or alcohol tests in Louisville, KY, or use our online registration system. Schedule by selecting your test and completing the Donor Information/Registration section. Registering ahead is necessary to visit the testing center.

Enter your zip code to find the nearest DOT testing centers in Louisville, KY. You'll get a donor pass with detailed instructions and center address via email. Bring this form or display it on your smartphone at the center. Completing the donor information and payment upfront is crucial, although most cases don't require prior appointments.

Our SAMHSA-certified labs provide reliable testing reviewed by our licensed MROs, ensuring accuracy and compliance with all legal standards.

In Louisville, KY, and its surrounding areas, Accredited Drug Testing offers comprehensive DOT drug and alcohol testing services with multiple testing centers available.

No matter where you are—be it on business or leisure—Accredited Drug Testing operates nationwide. We can set up your test, anytime, anyplace!

Kentucky Dept. of Transportation Updates

Louisville, KY DUI Statistics

Driving under the influence (DUI) remains a significant concern for Louisville, KY, and Jefferson County, where the city is located. In the latest collected data, DUI incidents have shown a concerning trend, impacting road safety and public health in the region. Kentucky's Department of Transportation (KYDOT) actively works to address DUI-related issues through awareness campaigns and stringent enforcement measures. In Louisville, the efforts to curb DUI incidents include increased patrols and sobriety checkpoints, reflecting the state's commitment to reducing these occurrences. Understanding the gravity of impaired driving, Kentucky (KY) emphasizes collaboration between law enforcement, the judicial system, and community organizations in Louisville to create safer roads for its residents.

Drug Involved Accidents in Jefferson County, KY

Drug-involved accidents present a significant challenge for Louisville, KY, and Jefferson County as they continue to affect road safety. The Department of Transportation in Kentucky (KYDOT) indicates that controlled substances, including both prescription medications and illicit drugs, contribute to a notable percentage of traffic incidents. In Louisville, where such accidents have increased, efforts are being made to enhance drivers' awareness of the dangers associated with drug-impairment. Law enforcement agencies in KY focus on drug recognition training and collaborate with health professionals to tackle this issue. The overarching goal remains to mitigate drug-related accidents through targeted initiatives, promoting responsible driving behaviors, and implementing preventive strategies within the region.

Marijuana Related Accidents in Louisville, KY

Marijuana-related accidents are an emerging concern in Louisville, KY, as the landscape of drug use evolves following recent legislative changes across the United States. Although marijuana legalization discussions have spurred debates, it remains crucial for Louisville and broader Jefferson County to monitor its implications on road safety. The KY Department of Transportation (KYDOT) observes that while marijuana is gaining social acceptance, its role in impaired driving incidents cannot be overlooked. In Louisville, initiatives aimed at reducing marijuana-related accidents involve community education programs and collaboration with advocacy groups to highlight the significance of refraining from driving under the influence. Kentucky's approach underscores a balanced strategy, addressing public safety in parallel with evolving drug policies.

Louisville Drug Testing

Quickly find trusted local drug testing centers in Louisville, KY — fast, convenient, and reliable every time!

Louisville DOT Drug Testing

Quickly find a local DOT drug testing center in Louisville, KY — fast, reliable, convenient nationwide service!

Louisville DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Louisville, KY.

Louisville Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Louisville, KY.v

Louisville Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Louisville, KY locations—results you can trust, every time!

Louisville Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Louisville, KY.

Louisville Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Louisville, KY.

Louisville 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Louisville, KY employers and individuals nationwide.

Louisville 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Louisville, KY workplace and personal testing needs.

Louisville On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Louisville, KY workplace compliant.

Louisville DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Louisville, KY drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

phone support has been excellent

I have used ADT several times. Their phone support has been excellent, both when putting in the orders and when asking for help to interpret results.

Tee Ess - 12/19/2024

Accredited has been wonderful to work with!

Accredited has been wonderful to work with! My court ordered tests are on a very strange schedule but every time I call, they are quick to schedule me and are always able to find a location that works for where I’m at and when! Thank you for making this rather undesirable situation less stressful and efficient.

Madison Sanderford - 8/19/2024

Very Fun and Easy

Trish last week and Tatiana this week, very fun and easy folks to deal with. Well be using them more and more in the future.

Tom O - 12/19/2024

Super helpful

Customer service was surprisingly super helpful and so nice. She was able to get me in at a local location right away the same day. She helped me figure out exactly what I needed. Very impressed.

Brook - 9/19/2024

Sweet and helpful

Trish was amazing and got me through the sytem very fast and swift. I had a hard time hearing her a couple of times, but she was super sweet and helpful throughout the process. Highly recommend her!

Sophia Schutze - 6/19/2024

Super Helpful

Detricia was super helpful in making sure that the drug test is sent my worker for was the right one I needed for my company to be compliant with my customers drug test requirements.

Kevin Howell - 12/19/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

Fast and easy

It was fast and easy a pleasure to talk to the customer service people great service

Peter Villegas - 1/19/2025

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025


(800) 221-4291