DOT Drug Testing - Frankfort, KY

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 40 testing centers in the Frankfort area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

40 DOT Drug-Testing Centers in Frankfort

Minutes Away, Test Today

859 East Main St Suite 2c

1.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
859 East Main St Suite 2c
Frankfort, KY 40601

4 Hmb Cir

2.36 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4 Hmb Cir
Frankfort, KY 40601

101 Medical Heights Dr Ste G

3.34 mi
🕑 Facility: Mon–Fri: 06:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 01:00 PM - 03:30 PM
101 Medical Heights Dr Ste G
Frankfort, KY 40601

1140 Lexington Rd

16.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1140 Lexington Rd
Georgetown, KY 40324

1150 Lexington Rd Ste 104

16.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1150 Lexington Rd Ste 104
Georgetown, KY 40324

1221 S Broadway

17.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1221 S Broadway
Lexington, KY 40504

1218 S Broadway Ste 310

17.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1218 S Broadway Ste 310
Lexington, KY 40504

60 Mack Walters Rd Rear Entrance

18.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
60 Mack Walters Rd Rear Entrance
Shelbyville, KY 40065

727 Hospital Dr

19.70 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-12:30 PM & 1:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
727 Hospital Dr
Shelbyville, KY 40065

101 Stonecrest Rd Ste 101

20.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 Stonecrest Rd Ste 101
Shelbyville, KY 40065

1722 Sharkey Way

20.82 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1722 Sharkey Way
Lexington, KY 40511

1 Saint Joseph Dr

20.98 mi
🕑 Facility: Mon–Fri: 07:00 AM - 06:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 12:30 PM, 01:30 PM - 04:30 PM
1 Saint Joseph Dr
Lexington, KY 40504

1055 Wellington Way Ste 170

21.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1055 Wellington Way Ste 170
Lexington, KY 40513

1000 Monarch St Ste 180

21.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1000 Monarch St Ste 180
Lexington, KY 40513

989 Governors Ln, Ste 140

21.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
989 Governors Ln, Ste 140
Lexington, KY 40513

2343 Alexandria Dr

21.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2343 Alexandria Dr
Lexington, KY 40504

1051 Newtown Pike Ste H-j

22.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1051 Newtown Pike Ste H-j
Lexington, KY 40511

1498 Boardwalk

23.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1498 Boardwalk
Lexington, KY 40511

225 Hospital Dr Ste 200a

23.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
225 Hospital Dr Ste 200a
Winchester, KY 40391

128 Southland Dr

23.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
128 Southland Dr
Lexington, KY 40503

500 W New Circle Rd

23.31 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
500 W New Circle Rd
Lexington, KY 40511

330 Roland Ave

23.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
330 Roland Ave
Owenton, KY 40359

173 E Brannon Rd

24.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
173 E Brannon Rd
Nicholasville, KY 40356

1055 Dove Run Rd

25.01 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1055 Dove Run Rd
Lexington, KY 40502

4071 Tates Creek Centre Drive Ste 202

25.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4071 Tates Creek Centre Drive Ste 202
Lexington, KY 40517

204 Bellaire Dr

25.93 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
204 Bellaire Dr
Nicholasville, KY 40356

1099 Duval St Ste 150

26.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1099 Duval St Ste 150
Lexington, KY 40515

1250 Keene Rd Ste 101

26.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1250 Keene Rd Ste 101
Nicholasville, KY 40356

1024 N Main St

26.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1024 N Main St
Nicholasville, KY 40356

2350 Grey Lag Way

26.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2350 Grey Lag Way
Lexington, KY 40509

3925 Frankfort Ford Rd

26.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3925 Frankfort Ford Rd
Wilmore, KY 40390

155 Prosperous Pl Ste 1a

27.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
155 Prosperous Pl Ste 1a
Lexington, KY 40509

117 Quinn Dr

27.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
117 Quinn Dr
Nicholasville, KY 40356

141 N Eagle Creek Dr Ste 103,

27.29 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
141 N Eagle Creek Dr Ste 103,
Lexington, KY 40509

2424 Sir Barton Way Ste 175

27.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2424 Sir Barton Way Ste 175
Lexington, KY 40509

3439 Buckhorn Dr

27.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3439 Buckhorn Dr
Lexington, KY 40515

3475 Richmond Rd #260

27.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3475 Richmond Rd #260
Lexington, KY 40509

1509 Louisville Rd

27.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1509 Louisville Rd
Harrodsburg, KY 40330

1210 Ky Highway 36 E

29.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1210 Ky Highway 36 E
Cynthiana, KY 41031

151 N Eagle Creek Dr

30.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
151 N Eagle Creek Dr
Lexington, KY 40509

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 40 DOT drug testing locations to serve you in and around Frankfort, KY.

Accredited Drug Testing offers comprehensive DOT Drug and Alcohol Testing solutions in Frankfort, KY, meeting 49 CFR Part 40 requirements for all DOT modes including FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Our services encompass DOT Physicals, Random Pool Consortium enrollment, MIS, MVR, and driver qualification reports. Centers are conveniently situated, providing rapid same-day service for both employers and operators.

Choose the specific DOT service or testing needed, and find a nearby center in Frankfort, KY. Our straightforward process simplifies the arrangement of DOT drug testing for individuals, employees, and other parties. Accessibility in Frankfort ensures ease and efficiency.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Frankfort, Kentucky DOT Services

DOT Drug Testing & Services Available in Frankfort, KY:

Accredited Drug Testing offers specialized DOT services in Frankfort, KY, facilitating required screenings for compliance. Benefit from our expert management of testing necessities ensuring regulatory adherence.

DOT Services in Frankfort, KY

DOT mandates regular drug and alcohol screenings to ensure employee safety, especially in safety-sensitive roles, in Frankfort, KY. These regulations help maintain a safe and productive work environment.

Employers in Frankfort, KY are required to implement DOT testing programs to stay compliant. Accredited Drug Testing helps enforce these policies through our comprehensive services.

Random testing, part of DOT regulations, ensures fairness and compliance among employees in Frankfort, KY, preventing impairment in work operations.

DOT's stringent rules are essential for public safety, especially in Frankfort, KY where many sectors rely on transportation services, necessitating rigorous testing protocols.

In Frankfort, Kentucky, understanding and enforcing DOT regulations can lead to a safer workforce, benefiting overall community safety and operational efficiency.

FMCSA Information

FMCSA requires mandatory drug screenings for commercial drivers in Frankfort, KY, aiming to eradicate impaired driving on roads.

Ensuring driver sobriety, FMCSA's rules are crucial in Frankfort, Kentucky for operational and public safety.

USCG Information

USCG requires drug testing for maritime personnel in Frankfort, KY, critical for safety and seamless operations on waterways.

The USCG's policies in Frankfort, Kentucky help maintain marine operation safety through diligent testing protocols.

FAA Information

FAA enforces drug testing rules to certify that aviation personnel in Frankfort, KY remain fit for duty, safeguarding aviation standards.

Maintaining aviation safety in Frankfort, Kentucky, FAA's stringent drug testing rules ensure experienced and unimpaired service.

FRA Information

The FRA mandates drug testing in Frankfort, KY ensuring safe and reliable railroad operations by keeping personnel unimpaired.

Ensuring efficient rail service in Frankfort, Kentucky, FRA's stringent regulations are integral for operational safety.

FTA Information

FTA facilitates regular testing to ensure public transit employees in Frankfort, KY operate safely and meet regulatory compliance.

In Frankfort, Kentucky, the FTA ensures public transit systems remain safe and comply with drug and alcohol testing laws.

PHMSA Information

PHMSA enforces strict testing in Frankfort, KY ensuring hazardous materials are handled safely and responsibly.

By ensuring safety, PHMSA's regulations help mitigate risks associated with transporting dangerous goods in Frankfort, KY.

Scheduling in Frankfort, KY

For scheduling DOT drug and alcohol tests in Frankfort, KY, contact us at (800) 221-4291 or use our online express system. Choose your test, complete the registration, and register before visiting the testing center.

Enter your zip code to locate the nearest DOT center in Frankfort, KY. A form with details of hours and location will be emailed to you. Bring this form or show it on your smartphone—no appointment is usually needed. Finish registration and payment to proceed.

Our SAMHSA-certified labs assure accurate results, verified by our licensed physicians acting as Medical Review Officers (MRO).

Accredited Drug Testing provides DOT testing across Frankfort, KY, with additional centers in nearby locations.

Whether traveling or on holiday, Accredited Drug Testing offers nationwide testing availability to schedule at your convenience, wherever you are.

Kentucky Dept. of Transportation Updates

DUI Statistics in Frankfort, KY

Frankfort, the capital city of Kentucky, has taken considerable measures to address the issue of driving under the influence (DUI). In Franklin County, which encompasses Frankfort, DUI cases have historically been a significant concern for the Kentucky Department of Transportation (KYDOT). Based on recent data, the county has implemented stricter enforcement measures, including DUI checkpoints and educational campaigns to reduce such incidents. In 2022, Franklin County reported a decrease in DUI-related accidents by approximately 10% compared to the previous year. The community continues to raise awareness about the dangers of impaired driving, working alongside the Kentucky DOT to ensure safer roads for residents and visitors alike. Kentucky's commitment to combating DUIs in Frankfort highlights an ongoing effort to promote responsible driving behaviors and enhance road safety.

Drug-Involved Accidents in Frankfort, KY

Drug-involved accidents have been an area of focus for Frankfort, KY, and the surrounding Franklin County, especially considering the broader national opioid crisis. The Kentucky Department of Transportation (KYDOT) tracks these incidents to identify trends and effective prevention strategies. Recent statistics indicate that drug-related accidents, which include the influence of prescription drugs and illegal substances, have shown a slight increase. Efforts to mitigate these incidents include collaboration with local law enforcement, healthcare providers, and community organizations to educate the public on the risks of drug-impaired driving. The KYDOT has also supported initiatives to ensure first responders are equipped to handle such situations more effectively. These programs strive to reduce the impact of drug-related accidents within Frankfort and contribute to statewide efforts in Kentucky.

Marijuana-Related Accidents in Frankfort, KY

As part of a nationwide discussion on marijuana legalization and its implications for traffic safety, Frankfort, KY, located in Franklin County, has monitored marijuana-related accidents with growing concern. While marijuana remains illegal for recreational use in Kentucky, there has been an observable impact on driving safety, as recorded by the Kentucky Department of Transportation (KYDOT). In recent years, Franklin County has experienced a moderate increase in accidents where marijuana was identified as a contributing factor. To address this, KYDOT and local law enforcement have emphasized preventative measures such as community outreach programs and educational campaigns about the dangers of driving under the influence of marijuana. These initiatives aim to inform motorists in Frankfort and throughout Kentucky about the potential consequences and enhance public safety on the roads.

Frankfort Drug Testing

Quickly find trusted local drug testing centers in Frankfort, KY — fast, convenient, and reliable every time!

Frankfort DOT Drug Testing

Quickly find a local DOT drug testing center in Frankfort, KY — fast, reliable, convenient nationwide service!

Frankfort DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Frankfort, KY.

Frankfort Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Frankfort, KY.v

Frankfort Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Frankfort, KY locations—results you can trust, every time!

Frankfort Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Frankfort, KY.

Frankfort Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Frankfort, KY.

Frankfort 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Frankfort, KY employers and individuals nationwide.

Frankfort 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Frankfort, KY workplace and personal testing needs.

Frankfort On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Frankfort, KY workplace compliant.

Frankfort DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Frankfort, KY drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

Super Helpful

Detricia was super helpful in making sure that the drug test is sent my worker for was the right one I needed for my company to be compliant with my customers drug test requirements.

Kevin Howell - 12/19/2024

Very professional and very quick

Very professional and very quick service to schedule appointments! I am pleased with the services and would definitely recommend using them for any drug testing needs!

Carrie Feerick - 4/19/2025

Quick, knowledgeable and courteous

Quick, knowledgeable and courteous Janelle worked diligently to support me. I am recommending their services to anyone looking.

Greensboro Joseph - 11/19/2024


(800) 221-4291